Genetic and Structural Analyses of the TMV Movement Protein

TMV 运动蛋白的遗传和结构分析

• 批准号: 9317368
• 负责人: Roger Beachy
• 金额: $ 33.18万
• 依托单位: The Scripps Research Institute
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 1997-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317368&HistoricalAwards=false
• 关键词: Genetic; Structural; Analyses; TMV; Movement

9317368 Beachy Many plant viruses direct the synthesis of one or more proteins required for the spread of infection from the initial; site to adjacent cells. In the case of tobacco mosaic virus the movement protein responsible for spread of the virus has been identified and extensively studied. Nevertheless, the cellular and molecular mechanism responsible for the spread are not well understood. Previous studies have suggested that the ability of the movement protein to facilitate virus movement is the result of a combination of several functions or characteristics: 1) association with plasma membranes; 2) association with cell walls; 3) association with plasmodesmata with or without the formation of fibrous material in the channel; 4) modification of the plasmodesmata size exclusion limit; 5) formation of large, soluble complex perhaps including RNA. The proposed research will determine which functions are required for biological activity. Complementation of nonfunctional mutants of the movement protein will be used to identify regions of the protein that may be responsible for separate functions. A combination of in vitro and in vivo studies will then be used to identify which functions are associated with the mutated sequences. %%% The results of the study on the function of the virus movement protein will lead to a better understanding of how viral infections spread in plants. In turn this may lead to novel methods in preventing plant diseases. In addition, because the movement protein interacts with plasmodesmata, the knowledge obtained will lead to a greater understanding of intercellular communication in plants ***

小行星9317368 许多植物病毒指导一种或多种蛋白质的合成，这些蛋白质是感染从最初的部位扩散到邻近细胞所必需的。 在烟草花叶病毒的情况下，负责病毒传播的运动蛋白已被鉴定和广泛研究。 然而，负责传播的细胞和分子机制还没有很好地理解。 以前的研究表明，运动蛋白促进病毒运动的能力是几种功能或特征组合的结果：1）与质膜结合; 2）与细胞壁结合; 3）与胞间连丝结合，在通道中形成或不形成纤维材料; 4）改变胞间连丝的尺寸排阻极限; 5）形成大的可溶性复合物，可能包括RNA。 拟议的研究将确定生物活性所需的功能。 运动蛋白的非功能突变体的互补将用于鉴定可能负责单独功能的蛋白质区域。 然后将使用体外和体内研究的组合来鉴定哪些功能与突变序列相关。 对病毒运动蛋白功能的研究结果将有助于更好地了解病毒感染如何在植物中传播。 反过来，这可能导致预防植物疾病的新方法。 此外，由于运动蛋白与胞间连丝相互作用，所获得的知识将使我们对植物细胞间通讯有更深入的了解 *