Mechanism of Disease Resistance in Transgenic Plants

转基因植物的抗病机制

• 批准号: 8610390
• 负责人: Roger Beachy
• 金额: $ 1.8万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1989-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8610390&HistoricalAwards=false
• 关键词: Mechanism; Disease; Resistance; Transgenic; Plants

Mechanism of Disease Resistance in Transgenic Plants: Dr. Beachy recently reported the production of transgenic tobacco plants (plants regenerated from cells into which foreign DNA was introduced by transformation) that expressed the coat protein (CP) gene of tobacco mosaic virus (TMV) as a chimeric nuclear gene. These plants and their progeny were less susceptible to infection by TMV, and the appearance of symptoms caused by infection were significantly delayed in the transgenic plants compared to control plants. In addition, some of the transgenic plants escaped infection. Studies are continuing to determine the mechanism(s) that confer resistance to these transgenic plants. These experiments include site directed mutations to alter the amino acid sequence of the TMV- CP, as well as its subcellular localization in transgenic plants. This action provides funds to purchase two pieces of equipment necessary for the successful completion of the work, specifically, a controlled enviornment incubator/shaker for bacterial cultures, and a controlled environment chamber for holding plants that are infected with virus.

转基因植物的抗病机制 比奇博士最近报道了转基因 烟草植物（从细胞再生的植物， 通过转化引入外源DNA）表达 烟草花叶病毒（TMV）的外壳蛋白（CP）基因作为一种 嵌合核基因 这些植物及其后代 不易受TMV感染， 感染引起的症状明显延迟 与对照植物相比，转基因植物。 此外，本发明还提供了一种方法， 一些转基因植物没有受到感染。 研究是 继续确定产生耐药性的机制 这些转基因植物。 这些实验包括 定向突变以改变TMV的氨基酸序列， CP及其在转基因植物中的亚细胞定位 植物 这一行动提供资金购买两件 成功完成工作所需的设备， 具体地，一种受控环境培养箱/振荡器， 细菌培养物，以及用于 种植着被病毒感染的植物。