Cellular and Molecular Studies on Plasmodemata

疟原虫的细胞和分子研究

• 批准号: 9209530
• 负责人: Roger Beachy
• 金额: $ 25.5万
• 依托单位: The Scripps Research Institute
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9209530&HistoricalAwards=false
• 关键词: Cellular; Molecular; Studies; Plasmodemata

This research will integrate biophysical, biochemical, cellular, physiological and genetic studies on plasmodesmata (pd) to establish an appropriate framework that will then allow an investigation of the cellular processes by which the plant regulates its cell-to-cell communication system. Biophysical studies will be performed on a simple cellular system in order to determine the unitary pd conductance. Such measurements are essential for a further sub-structural analysis of the pd, as well as to provide a rigorous foundation for detailed studies on the cellular controls that orchestrate cell-to-cell size exclusion limits. Physiological studies on the role of pd in solute fluxes will also be performed. Since pd are likely to be an important determinant of organ development, an experimental system will be established that will permit identification of the processes that control secondary pd formation. In this ongoing collaboration (between Beachy and Dr. William Lucas of the University of California, Davis), it has been established that transgenic tobacco plants in which the 30 kDa movement protein (MP) of TMV is expressed have pd whose size exclusion limits are up-regulated to sizes in excess of 9.4 kDa. The mode by which the 30 kDa TMV-MP effects this change will be further explored. It is hypothesized that this protein interacts directly with the plasmodesma. Based on this concept, biochemical isolation and purification of pd proteins are proposed. A genetic system in Arabidopsis will be developed to permit the identification and isolation of genes involved in virus movement and pd function. %%% Plasmodesmata are microscopic channels between individual plant cells. Intercellular communication by passage of molecules through these channels is critical for the normal development and physiological function of the entire plant. In certain plant viral infections, the virus gains access to the cell interior at a localized site and passes from cell to cell through the plasmodesmata throughout the plant. Passage of the virus through plasmodesmata is facilitated by a virally encoded protein which effectively enlarges the plasmodesmatal channel. This research will take advantage of the viral protein to identify and study the components of the plant plasmodesmata. Knowledge of the components of plasmodesmata will facilitate further studies of the formation of plasmodesmata and the mechanisms by which molecular traffic through the plasmodesmata is regulated. The results of this research will be important contributions to basic knowledge of cell to cell communication in plants.

这项研究将整合生物物理，生物化学， 胞间连丝的细胞、生理和遗传学研究 建立一个适当的框架， 研究植物生长的细胞过程 调节细胞间的通讯系统 生物物理 将在简单的蜂窝系统上进行研究， 确定单位Pd电导。 这样的测量 对于进一步的PD子结构分析也是必要的， 以提供一个严格的基础， 协调细胞间大小排阻的细胞控制 限制. pd在溶质通量中作用的生理学研究 也将被执行。 由于PD可能是一个重要的 器官发育的决定因素，实验系统将 建立的，将允许识别的过程， 控制二次钯的形成。 在这场持续的合作中， （比奇和威廉·卢卡斯博士之间的大学。 加州，戴维斯），已经确定，转基因烟草 其中TMV的30 kDa运动蛋白（MP）被 表达具有其大小排阻限度被上调至 分子量超过9.4kDa。 30 kDa TMV-MP的模式 将进一步探讨这一变化的影响。 据推测 这种蛋白质直接与胞间连丝相互作用。 基于 在此基础上，生化分离和纯化pd 蛋白质提出。 拟南芥的遗传系统将 它的发展是为了识别和分离基因， 参与病毒运动和PD功能。 %%% 胞间连丝是植物个体之间的微观通道 细胞 细胞间通讯通过分子通过 这些渠道对正常发展至关重要， 整个植物的生理功能。 在某些植物病毒中 感染时，病毒以一定的速度进入细胞内部。 局部化的位点，并通过细胞从细胞到细胞 整个植物的胞间连丝。 病毒通过 胞间连丝由病毒编码的蛋白质促进， 有效地扩大了胞间连丝通道。 本研究 将利用病毒蛋白来鉴定和研究 植物胞间连丝的组成部分。 组件知识 胞间连丝的形成将有助于进一步研究 以及分子运输的机制 通过胞间连丝进行调节。 的结果 研究将对细胞的基础知识做出重要贡献 to cell细胞communication通信in plants植物.