Cellular and Molecular Studies of Tobamovirus Movement Proteins

烟草花叶病毒运动蛋白的细胞和分子研究

• 批准号: 9631124
• 负责人: Roger Beachy
• 金额: $ 33万
• 依托单位: The Scripps Research Institute
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9631124&HistoricalAwards=false
• 关键词: Cellular; Molecular; Studies; Tobamovirus; Movement

96-31124 Beachy Most plant viruses, including tobacco mosaic virus (TMV), use plasmodesmata, the cytoplasmic bridges between proximal cells, to spread to adjacent cells. Most plant viruses encode one or two proteins that are required for virus movement through the plasmodesmata. These proteins are termed movement proteins (MP). The latter apparently functions by modifying the size exclusion limits of plasmodesmata. The PI has constructed mutants of MP in which the latter is fused to the green fluorescent protein (GFP) of Aequorea victoria to study cellular distribution and mode of action of the MP. The general goals of this proposal are: to determine the time course of GFP-MP cellular distribution during the course of infection in protoplasts and in leaves; to create mutations in the MP that leads to accumulation of GFP-MP in specific cellular structures, but not in other; to isolate cellular structures that contain GFP-MP, to determine what cellular components are associated with GFP-MP; as well as studies that include detailed analyses of GFP-MP in cells. These studies will add significantly to our knowledge about the intra- and inter-cellular distribution of TMV, as well as our knowledge of the role of the cytoskeleton in the targeting and transport of proteins in plasmodesmata. %%% Most plant viruses have evolved the capacity to use components in host cells for viral genome replication, for translation of viral proteins, for sorting and packaging of viral components, for export, and for spreading to proximal cells. For the latter process, most viruses encode proteins that modify the size exclusion limit of plasmodesmata. These proteins are termed movement proteins (MP). Little is known about the molecular mechanism of the MP. The proposed studies will investigate the cellular localization and mode of action of the tobacco mosaic virus (TMV) MP. These studies will increase significantly our knowledge about the cellular distribution of TMV as well as the role of the cytoskeleton in the t argeting and, possibly, in the transport of MP to plasmodesmata. ***

96-31124比奇 大多数植物病毒，包括烟草花叶病毒（TMV），使用胞间连丝，近端细胞之间的细胞质桥梁，传播到相邻的细胞。大多数植物病毒编码一种或两种蛋白质，这些蛋白质是病毒通过胞间连丝运动所必需的。这些蛋白质被称为运动蛋白（MP）。后者显然是通过修改胞间连丝的大小排阻限制发挥作用的。PI已经构建了MP的突变体，其中后者与维多利亚水母的绿色荧光蛋白（GFP）融合，以研究MP的细胞分布和作用模式。该建议的总体目标是：确定在原生质体和叶中感染过程中GFP-MP细胞分布的时间过程;在MP中产生导致GFP-MP在特定细胞结构中而不是在其他细胞结构中积累的突变;分离含有GFP-MP的细胞结构，以确定哪些细胞组分与GFP-MP相关;以及包括细胞中GFP-MP的详细分析的研究。这些研究将大大增加我们对TMV的细胞内和细胞间分布的了解，以及我们对细胞骨架在胞间连丝蛋白质的靶向和运输中的作用的了解。 大多数植物病毒已经进化出利用宿主细胞中的组分进行病毒基因组复制、翻译病毒蛋白、分选和包装病毒组分、输出以及传播到近端细胞的能力。对于后一个过程，大多数病毒编码的蛋白质改变胞间连丝的大小排阻限制。这些蛋白质被称为运动蛋白（MP）。关于MP的分子机制知之甚少。拟开展的研究将调查烟草花叶病毒（TMV）MP的细胞定位和作用方式。这些研究将大大增加我们对TMV的细胞分布以及细胞骨架在MP向胞间连丝运输中的作用的认识。 ***