RUI: Gene Flow and Hybridization in Milkweeds (Asclepias L.) A Comparison of Two Sympatric Species

RUI：马利筋（Asclepias L.）的基因流和杂交两个同域物种的比较

• 批准号: 9317476
• 负责人: Steven Broyles
• 金额: $ 6.57万
• 依托单位: SUNY College at Cortland
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1997-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317476&HistoricalAwards=false
• 关键词: RUI; Gene; Flow; Hybridization; Milkweeds

WPC 2 B V P Z Courier 10cpi ? x x x , Z x 6 X @ 8 ; X @ HP DeskJet 500 HPDES500.PRS x @ x X , , 0 FX @Courier 10cpi 2 @ V P Z #| x ? x x x , Z x 6 X @ 8 ; X @ HP DeskJet 500 HPDES500.PRS x @ x X , , 0 FX @ 9317476 Broyles Hybridization and long distance pollen movement (i.e. gene flow) are important processes that are responsible for introducing and maintaining genetic diversity within plant populations. In the proposed study, I will examine pollen dispersal, gene flow, and effective hybridization between two common North American milkweeds (Poke Milkweed, Asclepias exaltata L.; Common Milkweed, Asclepias syriaca L.). These species occur in the same areas and overlap in flowering time, share pollinators, form viable hybrids, maintain high levels of genetic variation, and possess several unique genetic markers. Together these features make them ideal candidates for examining gene flow and hybridization. Nevertheless, A . exaltata and A . syriaca differ in several reproductive features (e.g., asexual reproduction, investment in pollen producing structures, and inflorescence size) which permits us to examine the effects of these life history traits on gene flow and hybridization. Specifically, gene flow and hybridization will be quantified by identifying pollen parents of seed to examine (1) pollen dispersal, (2) levels of long distance pollen mediated gene flow, and (3) effective hybridization between these species. The results of this study will contribute to our understanding of gene flow and hybridization in the maintenance of genetic diversity in plant populations. These processes are important to study because genetic var iation provides the necessary material for adaptation and long term species conservation. I will examine the underlying pollination mechanisms and plant pollinator interactions that directly affect these evolutionary processes. In addition, this study should lay the foundation for more comprehensive studies on the processes and evolutionary consequences of long distance pollen dispersal and hybridization in natural populations.

WPC 2 B V P Z 10cpi ? x x x， Z X 6 X @ 8 ; X @ HP DeskJet 500 HPDES500.PRS X @ x X，，0 FX @ CPI 10cpi 2 @ V P Z #|X ? x x x， Z X 6 X @ 8 ; X @ HP DeskJet 500 HPDES500.PRS X @ x X，，0 FX@9317476 Broyles 杂交和花粉远距离移动（即基因流）是植物种群遗传多样性导入和维持的重要过程。 在拟议的研究中，我将研究两种常见的北美乳草（Poke Milkweed， Asclepias 埃克萨勒塔塔 L.的;普通乳草， Asclepias syriaca L.）。 这些物种出现在同一地区，开花时间重叠，共享传粉者，形成可行的杂交种，保持高水平的遗传变异，并拥有几个独特的遗传标记。 这些特征使它们成为研究基因流动和杂交的理想候选者。 然而，尽管如此， 一 . 埃克萨勒塔塔 和 一 . syriaca 在几个生殖特征上存在差异（例如，无性繁殖、花粉产生结构的投资和花序大小），这使我们能够研究这些生活史性状对基因流动和杂交的影响。 具体而言，将通过鉴定种子的花粉亲本来定量基因流动和杂交，以检查（1）花粉传播，（2）远距离花粉介导的基因流动的水平，和（3）这些物种之间的有效杂交。 本研究的结果将有助于我们理解基因流和杂交在植物种群遗传多样性的维持。 这些过程是重要的研究，因为遗传变异提供了必要的材料，适应和长期的物种保护。 我将研究潜在的授粉机制和植物传粉者的相互作用，直接影响这些进化过程。 此外，本研究还为更全面地研究自然居群中远距离花粉传播和杂交的过程和进化结果奠定了基础。