Pattern Formation in Plants: Biophysics and Gene Expression

植物模式形成：生物物理学和基因表达

• 批准号: 9317755
• 负责人: Paul Green
• 金额: $ 30万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-15 至 1997-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317755&HistoricalAwards=false
• 关键词: Pattern; Formation; Plants; Biophysics; Gene

9317755 Green In the normal development of flowers and vegetative shoots of plants, there is a highly coordinated pattern of gene expression and morphological change. Dr. Green will study both processes and their mode of interaction. Expression gradients are postulated to set up physical expansion gradients which lead to the spontaneous complex folding of tissue. This is a new proposal for the origin of periodicity and pattern. The morphological change is postulated to act back on expression via stretch receptors or cytoskeletal responses. This completes a generative cycle for morphogenesis. Organ pattern on the sunflower head emerges in strict developmental sequence behind a circular generative zone which moves from the rim into a central field of undifferentiated cells. The major aim is to determine the relative sequence of expression events and of folding events in this meristem. Pattern and tissue folding are to be followed in detail by a non-destructive SEM replica method. Expression is to be studied by in situ hybridization. Similar analysis is proposed for other systems, including morphological mutants. Dr. Green will study changes brought on by non-invasive physical perturbation. The nature of the cyclic interaction of expression and morphological response should become clear. ***

9317755绿色在植物花和营养芽的正常发育过程中，存在着一个高度协调的基因表达和形态变化模式。格林博士将研究这两个过程及其相互作用的模式。假设表达梯度建立了导致组织自发复杂折叠的物理膨胀梯度。这是对周期性和模式起源的一种新的认识。假设形态变化通过拉伸受体或细胞骨架反应作用于表达。这就完成了形态发生的一个生殖周期。太阳花头部的器官图案是在一个圆形的生殖区后面严格的发育顺序出现的，这个生殖区从边缘移动到未分化细胞的中心区域。主要目的是确定该分生组织中表达事件和折叠事件的相对顺序。图案和组织折叠将通过非破坏性扫描电镜复制方法详细跟踪。表达将通过原位杂交研究。类似的分析也适用于其他系统，包括形态突变体。格林博士将研究非侵入性物理扰动带来的变化。表达和形态反应的循环相互作用的性质应该变得清楚。＊＊＊