Pattern Formation in Plants: Biophysics and Gene Expression

9317755绿色在植物花朵和营养枝的正常发育中，基因表达和形态变化具有高度协调的模式。格林博士将研究这两个过程及其相互作用模式。表达梯度被假定为建立物理扩展梯度，从而导致组织的自发复杂折叠。这是对周期和模式起源的一个新的建议。这种形态变化被认为是通过伸展受体或细胞骨架反应对表达产生反作用。这就完成了形态发生的生殖循环。向日葵花冠上的器官模式以严格的发育顺序出现在圆形生殖区后面，生殖区从边缘进入未分化细胞的中心区域。主要目的是确定该分生组织中表达事件和折叠事件的相对顺序。图案和组织折叠将通过非破坏性的扫描电子显微镜复制法详细进行。表达将通过原位杂交进行研究。对包括形态突变体在内的其他系统也提出了类似的分析。格林博士将研究非侵入性物理扰动带来的变化。表达和形态反应的循环相互作用的本质应该变得清晰。***

9317755 Green In the normal development of flowers and vegetative shoots of plants, there is a highly coordinated pattern of gene expression and morphological change. Dr. Green will study both processes and their mode of interaction. Expression gradients are postulated to set up physical expansion gradients which lead to the spontaneous complex folding of tissue. This is a new proposal for the origin of periodicity and pattern. The morphological change is postulated to act back on expression via stretch receptors or cytoskeletal responses. This completes a generative cycle for morphogenesis. Organ pattern on the sunflower head emerges in strict developmental sequence behind a circular generative zone which moves from the rim into a central field of undifferentiated cells. The major aim is to determine the relative sequence of expression events and of folding events in this meristem. Pattern and tissue folding are to be followed in detail by a non-destructive SEM replica method. Expression is to be studied by in situ hybridization. Similar analysis is proposed for other systems, including morphological mutants. Dr. Green will study changes brought on by non-invasive physical perturbation. The nature of the cyclic interaction of expression and morphological response should become clear. ***