Acquisition of a Triple Resonance 500 MHz NMR Console

获取三重共振 500 MHz NMR 控制台

• 批准号: 9317898
• 负责人: Erik Zuiderweg
• 金额: $ 7.5万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1996-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317898&HistoricalAwards=false
• 关键词: Acquisition; Triple; Resonance; 500; MHz

9317898 Zuiderweg This proposal is a request for funds to purchase a new radio-frequency console, probes and accessories for an existing older 500 MHz NMR spectrometer. The upgraded system, which is actually on loan at the University of Michigan now, provides state-of-the-art multi-dimensional multi-nuclear magnetic resonance support for research supported faculty in the Biophysics Research Division and the Department of Biological Chemistry at the University of Michigan. The faculty involved is generally researching biological systems of molecular size exceeding 15 kDa. Because of this size, NMR support for their research will exclusively rely on advanced and time-consuming experiments in two, three and four dimensions involving up to four nuclei simultaneously with isotopically enriched materials, necessitating the upgrade. The purchase of the upgraded instrument will immediately benefit the research of seven faculty who hold a total of 16 active federally-sponsored research grants, one institutional training grant and two pending grants. Specific projects are: NMR structure determination of stromelysin catalytic domain NMR studies of Yersinia Phosphatase in Solution Determination of a high-resolution solution structure of T4-lysozyme by Multi-dimensional NMR NMR studies of the structures of catalytic RNA subdomains Studies of the interaction of a2 adrenergic receptor peptides with G-protein subtypes Electron Transfer and conformational gating in Flavodoxins: NMR analysis of E. coli Flavodoxin Methodology development in multi-nuclear multi- dimensional NMR

9317898 Zuiderweg 该提案是一项资金请求，用于为现有的旧 500 MHz NMR 波谱仪购买新的射频控制台、探头和配件。升级后的系统现在实际上是在密歇根大学借用的，它为密歇根大学生物物理研究部和生物化学系的研究支持教师提供最先进的多维多核磁共振支持。参与的教师通常研究分子大小超过 15 kDa 的生物系统。 由于这种尺寸，核磁共振对其研究的支持将完全依赖于二维、三维和四个维度的先进且耗时的实验，涉及最多四个核同时使用同位素富集材料，因此需要升级。购买升级后的仪器将立即有利于 7 名教师的研究，他们总共拥有 16 项联邦资助的有效研究补助金、一项机构培训补助金和两项待定补助金。 具体项目有： 溶基质素催化结构域的 NMR 结构测定 溶液中耶尔森氏菌磷酸酶的 NMR 研究 通过多维 NMR 测定 T4-溶菌酶的高分辨率溶液结构 催化 RNA 子结构域结构的 NMR 研究 研究 a2 肾上腺素受体肽与 G 蛋白亚型的相互作用 电子转移和构象门控 黄素氧还蛋白：大肠杆菌黄素氧还蛋白的 NMR 分析 多核多维 NMR 的方法学开发