Acquisition of a Triple Resonance 500 MHz NMR Console

该提案要求为现有的老式500 MHz核磁共振光谱仪购买新的射频控制台、探头和附件。升级后的系统，实际上是在密歇根大学租借的，为密歇根大学生物物理研究部和生物化学系的研究支持教师提供最先进的多维多核磁共振支持。所涉及的教师一般是研究分子大小超过15 kDa的生物系统。由于这种规模，核磁共振支持他们的研究将完全依赖于先进和耗时的实验，在二、三、四维，涉及多达四个核同时与同位素富集的材料，需要升级。购买升级后的仪器将立即使7个学院的研究受益，他们总共拥有16项联邦资助的研究补助金，一项机构培训补助金和两项未决补助金。具体项目有：醇解素催化结构域的核磁共振结构测定溶液中耶尔森氏菌磷酸酶的核磁共振研究通过多维核磁共振测定t4溶菌酶的高分辨率溶液结构催化RNA亚结构域的核磁共振结构研究a2肾上腺素能受体肽与g蛋白亚型的相互作用研究黄酮类毒素中的电子转移和构象门控核磁共振分析大肠杆菌黄氧多酚的方法在多核多维核磁共振中的发展

9317898 Zuiderweg This proposal is a request for funds to purchase a new radio-frequency console, probes and accessories for an existing older 500 MHz NMR spectrometer. The upgraded system, which is actually on loan at the University of Michigan now, provides state-of-the-art multi-dimensional multi-nuclear magnetic resonance support for research supported faculty in the Biophysics Research Division and the Department of Biological Chemistry at the University of Michigan. The faculty involved is generally researching biological systems of molecular size exceeding 15 kDa. Because of this size, NMR support for their research will exclusively rely on advanced and time-consuming experiments in two, three and four dimensions involving up to four nuclei simultaneously with isotopically enriched materials, necessitating the upgrade. The purchase of the upgraded instrument will immediately benefit the research of seven faculty who hold a total of 16 active federally-sponsored research grants, one institutional training grant and two pending grants. Specific projects are: NMR structure determination of stromelysin catalytic domain NMR studies of Yersinia Phosphatase in Solution Determination of a high-resolution solution structure of T4-lysozyme by Multi-dimensional NMR NMR studies of the structures of catalytic RNA subdomains Studies of the interaction of a2 adrenergic receptor peptides with G-protein subtypes Electron Transfer and conformational gating in Flavodoxins: NMR analysis of E. coli Flavodoxin Methodology development in multi-nuclear multi- dimensional NMR