High Field NMR Spectrometer

该奖项将支持购买750 MHz多核高分辨率NMR系统，主要用于密歇根大学化学/生物化学NMR社区。 该仪器由NSF和NIH通过NSF/NIH共享仪器计划共同资助。 该仪器将用于实验，导致确定高分辨率的三维结构和蛋白质，核酸和蛋白质-核酸复合物在溶液中的动力学。 新仪器的主要优点是，与目前可用的仪器相比，灵敏度至少提高了两倍，从而可以对这些更困难的系统进行结构研究。 从此次收购中受益的项目包括：分子伴侣蛋白Hsc-70和DnaK的结构研究，基质分解素及其与抑制剂和底物的复合物的结构研究，大肠杆菌表达的蛋白质的结构研究。coli Ars-操纵子，胞苷酰转移酶的结构解析，半胱氨酸蛋白酶抑制剂的设计，改进的DNA结合碳水化合物的合成，DNA三螺旋的动力学，抗DNA自身抗体抗原相互作用的解析，以及GABA受体的可溶性和跨膜结构域的结构。

This award will support the purchase of a 750 MHz multi- nuclear high-resolution NMR system primarily for the use of the University of Michigan Chemistry/Biological Chemistry NMR community. This instrument is jointly funded by NSF and NIH through the NSF/NIH Shared Instrumentation Program. The instrument will be used for experiments leading to the determination of high-resolution three-dimensional structures and dynamics of proteins, nucleic acids, and protein-nucleic acid complexes in solution. The major virtue of the new instrument is an improvement of at least a factor of two in sensitivity as compared to currently accessible instruments, bringing the structural studies of these more difficult systems within reach. Projects benefiting from this acquisition are: structural studies of the molecular chaperone proteins Hsc-70 and DnaK, structural studies of Stromelysin and its complexes with inhibitors and substrate, structural studies of proteins expressed by the E. coli Ars-Operon, structural elucidation of Cytidylyltransferase, design of Cysteine Protease inhibitors, synthesis of improved DNA binding carbohydrates, dynamics of DNA triple helices, elucidation of anti-DNA autoantibody antigen interactions, and the structure of soluble and membrane-spanning domains of the GABA receptor.