Acquisition of a Confocal Microscope for the Levine Science Resource Center

这是一个多用户共聚焦显微镜和配套仪器的建议。杜克大学的核心用户群包括化学系、动物学、细胞生物学、免疫学以及细胞生长、调节和癌症部门的教员。1994年，将占用一座新楼，即科学资源中心，目前正在作为一个多学科科学设施建造。该建筑将容纳环境学院，以及工程学院、艺术与科学学院和医学院的组成部分。该建筑的核心设施之一将是显微成像中心。该设施和大楼中所有其他共享用户技术的目的是使硬件和软件保持在生物技术的前沿，并找到将这些技术与其他新兴领域的进步相结合的方法。新大楼还计划建立一个核磁共振中心、x射线晶体学和蛋白质建模中心，以及该大学的转基因小鼠中心。每个中心都将共享用户操作，这些操作将相互补充，并将与显微成像中心交互。随着化学家、生物物理学家、物理学家、生物化学家、生物学家和计算机科学家在这些技术的持续发展中相互作用，这些中心将促进新的发展。这个共聚焦显微镜的应用程序汇集了来自五个部门的七个教员作为核心用户。我们每个人都用过共聚焦显微镜，不是在其他机构（我们中的四个是杜克大学的新教员），就是在杜克大学。目前，该中心有一台共聚焦显微镜，使用频率越来越高。这台显微镜需要升级，它本身已经不能支持许多被提议的应用了。因此，本请求是为了部分支持升级现有显微镜和购买新一代共焦显微镜以及支持硬件和软件的努力。我们的项目种类繁多。新的创新，加上用户流量的增加，将需要一个新的微观平台和新的可用激光源，再加上将建立在新建筑中的网络功能，将促进它们。

This proposal is for a multi-user confocal microscope and supporting instrumentation. The core user group at Duke University include faculty in the Department of Chemistry, Zoology, Cell Biology, Immunology, and the Section of Cell Growth, Regulation and Cancer. In 1994, a new building, the Science Resource Center, now being built as a multidisciplinary science facility, will be occupied. The building will house the School of the Environment, and components of the Schools of Engineering, Arts and Sciences, and Medicine. One of the core facilities in that building will be an microscopic Imaging Center. The intent for that facility, and all other shared-user technologies in the building, is to keep the hardware and software at the leading edge of biotechnology and to find ways to interface these technologies with advances in other emerging areas. Also being planned for the new building are an NMR center, X-ray crystallography and protein modeling Center, and the University's transgenic mouse Center. Each of these Centers will be shared user operations which will complement one another and will interface with the Microscopic Imaging Center. These Centers will foster new developments as the chemists, biophysicists, physicists, biochemists, biologists, and computer scientists interact with one another in the continuing development of these technologies. This application for a confocal microscope brings together seven faculty from five departments as the core users. Each of us has used confocal microscopes, either at other institutions (four of us are new faculty at Duke), or at Duke. At present the Center has one confocal microscope that has been getting increasingly heavier use. That microscope is in need of an upgrade, and it is no longer, by itself, capable of supporting many of the applications that are proposed. Therefore, this request is for partial support of an effort to upgrade the existing microscope and to purchase a newer generation of confoc al microscope plus support hardware and software. Our projects are quite varied. New innovations, plus the increase in traffic of users will require a new microscopic platform and newly available laser sources, plus they will be facilitated by the networking capabilities that will be build into the new building.