Adaptation of Insect Cells to Suspension Growth

9318670 Murhammer本项目旨在研究悬浮培养物对昆虫细胞/杆状病毒表达系统的生长和产物形成的影响。 许多不容易适应悬浮生长的昆虫细胞系具有比容易适应悬浮生长的细胞系显著更高的产物表达水平。 这一现象将通过以下方式进行研究：（1）确定悬浮培养物对未感染昆虫细胞的影响;（2）研究悬浮生长的遗传基础;（3）确定悬浮培养物对病毒感染过程的影响。 该项目的结果是提供必要的信息，以提高昆虫细胞培养的规模，其中重组蛋白和病毒杀虫剂的产量最大化。 ***

9318670 Murhammer This project is to study the effects of suspension cultures on the growth and product formation of insect cell/baculovirus expression systems. Many insect cell lines that are not easily adapted to suspension growth have significantly higher product expression levels than cell lines that are easily adapted to suspension growth. This phenomenon is to be investigated by: (1) determining the effect of suspension cultures on uninfected insect cells; (2) investigating the genetic basis of suspension growth; and (3) determining the effect of suspension cultures on the viral infection process. The results of this project are to provide information necessary for the improvement of insect cell culture scale-up in which the yields of recombinant proteins and viral insecticides are maximized. ***