A Diffusion Gradient System for Isolating Microorganisms and Studying Microbial Processes Related to Detoxification of Hazardous Chemicals

用于分离微生物和研究与危险化学品解毒相关的微生物过程的扩散梯度系统

• 批准号: 9361116
• 负责人: John Koh
• 金额: $ 6.47万
• 依托单位: Koh Development Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-15 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9361116&HistoricalAwards=false
• 关键词: Diffusion; Gradient; System; Isolating; Microorganisms

9361116 Koh Microorganisms are primarily responsible for the degradation of toxic chemicals that enter the environment. Successful isolation and exploitation of these microbes for bioremediation requires identifying the physico-chemical conditions under which detoxification activity is maximized. This work proposes to develop an analytical diffusion gradient chamber that mimics the diffusive processes that prevail in the microbial world. This system can be used to separate, enrich for, and isolate detoxifying microorganisms, as well as study the behavior of , and detoxification processes mediated by, these organisms. The proposed system will consist of a gradient chamber for culturing microorganisms in multiple, continuous diffusion gradients of small soluble molecules. In addition there will be an effluent chamber for consolidating effluent flow from the system, an environmental control unit of maintaining constant temperature, and an imaging unit to monitor and quantify microbial growth and movement. These components will be developed by Koh Development, Inc. The system will be tested and evaluated by Koh Development with help from researchers at Michigan State University (MSU). The scientists at MSU will also use the system to evaluate the chemotactic response of an individual bacterial species to multiple chemical cues. In addition, gradients of salt and toluene will be used to evaluate the response of a natural community from a soil site contaminated with oil brine. Mathematical models will also be developed that aid in optimizing the performance of the system.

9361116 Koh微生物主要负责降解进入环境的有毒化学物质。 成功分离和利用这些微生物进行生物修复需要确定最大限度地发挥解毒活性的物理化学条件。 这项工作提出了开发一个分析扩散梯度室，模仿扩散过程中流行的微生物世界。 该系统可用于分离、富集和分离解毒微生物，以及研究这些生物体的行为和介导的解毒过程。 所提出的系统将由用于在可溶性小分子的多个连续扩散梯度中培养微生物的梯度室组成。 此外，将有一个污水室，用于合并来自系统的污水流，一个保持恒温的环境控制单元，以及一个监测和量化微生物生长和运动的成像单元。 这些组件将由Koh Development，Inc.开发。 该系统将由Koh Development在密歇根州立大学（MSU）研究人员的帮助下进行测试和评估。 密歇根州立大学的科学家还将使用该系统来评估单个细菌物种对多种化学线索的趋化反应。 此外，盐和甲苯的梯度将被用来评估一个自然群落的反应，从土壤中的石油盐水污染的网站。 还将开发有助于优化系统性能的数学模型。