Production of Secondary Metabolites of Azadirachta Indica

9361664 Swedlund该项目的目标是为印楝树（Azadirachta indica）开发一种组织培养系统，以提供纯次生代谢物的来源。这种植物最著名的产品是杀虫剂印楝素。这种化合物是有效的，尽管它的毒性很低。在组织培养中生产印楝素将提供全年稳定的供应，同时消除了从种子材料中提取这种化合物所需的大量溶剂。第一阶段的研究目标包括：(1)确定各种激素对培养起始和维持的影响，以及印楝树细胞产生印楝素的影响；(2)利用最佳培养物进一步提高印楝素水平；(3)确定印楝种子中产生印楝素的位置，以便为组织培养培养更好的细胞类型。＊＊＊

9361664 Swedlund The goal of this project is to develop a tissue culture system for the neem tree, Azadirachta indica, to provide a source of pure secondary metabolites. The best known product from this plant is the insecticide azadirachtin. This compound is effective, even though it has a very low toxicity. Production of azadirachtin in tissue culture would provide a constant supply year round, while eliminating the large quantities of solvents that are required when this compound is obtained by extraction from seed material. The phase I research objectives include: (1) the determination of the effects of various hormones on the initiation and maintenance of cultures, and the production of azadirachtin by cells of the neem tree; (2) the use of the best cultures in further experiments designed to increase the levels of azadirachtin; and (3) to determine the location within neem seeds where azadirachtin is produced in order to develop better cell types for tissue culture. ***