Production of Secondary Metabolites of Azadirachta Indica

印楝次生代谢产物的生产

• 批准号: 9361664
• 负责人: Brad Swedlund
• 金额: $ 6.35万
• 依托单位: AgriDyne Technologies Incorporated (NPI)
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-01-15 至 1994-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9361664&HistoricalAwards=false
• 关键词: Production; Secondary; Metabolites; Azadirachta; Indica

9361664 Swedlund The goal of this project is to develop a tissue culture system for the neem tree, Azadirachta indica, to provide a source of pure secondary metabolites. The best known product from this plant is the insecticide azadirachtin. This compound is effective, even though it has a very low toxicity. Production of azadirachtin in tissue culture would provide a constant supply year round, while eliminating the large quantities of solvents that are required when this compound is obtained by extraction from seed material. The phase I research objectives include: (1) the determination of the effects of various hormones on the initiation and maintenance of cultures, and the production of azadirachtin by cells of the neem tree; (2) the use of the best cultures in further experiments designed to increase the levels of azadirachtin; and (3) to determine the location within neem seeds where azadirachtin is produced in order to develop better cell types for tissue culture. ***

9361664 Swedlund该项目的目标是为印楝树开发一种组织培养系统，以提供纯净的次生代谢物来源。这种植物最著名的产品是杀虫剂印木素。这种化合物是有效的，尽管它的毒性很低。在组织培养中生产印楝素将提供全年不变的供应，同时消除了从种子材料中提取这种化合物时所需的大量溶剂。第一阶段的研究目标包括：(1)确定各种激素对印楝树细胞培养的启动和维持以及印木素的产生的影响；(2)将最好的培养物用于进一步的实验，以提高印木素的水平；以及(3)确定印木种子中产生印木素的位置，以便为组织培养发展更好的细胞类型。***