NSF-CGP Science Fellowship Program: Cloning and Characterization of a cDNA for a Nuclear Protein that Binds to the 5' Upstream Region of a Rice Glutelin Gene

9402501 De Lumen该奖项资助加州大学伯克利分校营养科学系Benito De Lumen博士与筑波国家农业生物资源研究所细胞生物系Fumio Takaiwa博士在日本进行11个月合作研究的旅费。这项研究将集中在转录水平上对基因表达的调控，从而导致水稻种子胚乳中组织和阶段特异性表达。Takaiwa博士和他的团队在克隆和鉴定一些大米谷蛋白基因方面取得了重大进展。德卢门博士将与Takaiwa博士合作，尝试克隆和测序一种编码与水稻谷蛋白基因5‘上游区域结合的核蛋白的cDNAs，然后建立该cdna的阶段特异性表达，最后将其与克隆的转录因子进行同源性比较。该项目在很大程度上依赖于Takaiwa博士在识别水稻谷蛋白基因中与核蛋白相互作用的上游DNA控制序列方面所做的基础工作。当德卢门博士回到他在伯克利的实验室时，这项工作应该会促进利用大豆中富含蛋氨酸的蛋白基因克隆类似的转录因子。该奖项是由日本基金会全球伙伴关系中心(CGP)根据NSF-CGP科学奖学金计划资助的。***

9402501 De Lumen This award supports the travel expenses for Dr. Benito De Lumen of the Department of Nutritional Sciences at the University of California at Berkeley to spend eleven months in Japan conducting collaborative research with Dr. Fumio Takaiwa, Department of Cell Biology at the National Institute of Agrobiological Resources in Tsukuba. The research will focus on the regulation of gene expression at the transcription level that results in tissue and stage-specific expression in rice seed endosperm. Dr. Takaiwa and his team have made significant progress in cloning and characterizing a number of rice glutelin genes. Working with Dr. Takaiwa, Dr. De Lumen will attempt to clone and sequence a cDNA encoding a nuclear protein that binds to the 5' upstream region of a rice glutelin gene, then to establish the stage-specific expression of this cDNA, and finally to compare its homology with cloned transcription factors. This project depends heavily on the groundwork established by Dr. Takaiwa in identifying upstream DNA control sequences in a rice glutelin gene that interact with nuclear proteins. This work should facilitate the cloning of similar transcription factors using methionine-rich protein genes from soybeans when Dr. De Lumen returns to his laboratory in Berkeley. This award is funded under the auspices of the Center for Global Partnership (CGP) of the Japan Foundation under the NSF-CGP Science Fellowship Program. ***