NSF-CGP Science Fellowship Program: Cloning and Characterization of a cDNA for a Nuclear Protein that Binds to the 5' Upstream Region of a Rice Glutelin Gene

9402501 De Lumen 该奖项用于资助加州大学伯克利分校营养科学系的Benito De Lumen博士前往日本与筑波国立农业生物资源研究所细胞生物学系的Fumio Takaiwa博士进行为期11个月的合作研究。该研究将集中在转录水平上的基因表达调控，导致水稻种子胚乳中的组织和阶段特异性表达。 Takaiwa博士和他的团队在克隆和表征一些水稻谷蛋白基因方面取得了重大进展。与Takaiwa博士合作，De Lumen博士将尝试克隆和测序编码与水稻谷蛋白基因5'上游区域结合的核蛋白的cDNA，然后建立该cDNA的阶段特异性表达，最后将其与克隆的转录因子进行同源性比较。 该项目在很大程度上依赖于Takaiwa博士在确定水稻谷蛋白基因中与核蛋白相互作用的上游DNA控制序列方面建立的基础。当德·卢蒙博士回到他在伯克利的实验室时，这项工作将有助于利用大豆中富含甲硫氨酸的蛋白质基因克隆类似的转录因子。 该奖项由日本基金会全球伙伴关系中心（CGP）根据NSF-CGP科学奖学金计划资助。 ***

9402501 De Lumen This award supports the travel expenses for Dr. Benito De Lumen of the Department of Nutritional Sciences at the University of California at Berkeley to spend eleven months in Japan conducting collaborative research with Dr. Fumio Takaiwa, Department of Cell Biology at the National Institute of Agrobiological Resources in Tsukuba. The research will focus on the regulation of gene expression at the transcription level that results in tissue and stage-specific expression in rice seed endosperm. Dr. Takaiwa and his team have made significant progress in cloning and characterizing a number of rice glutelin genes. Working with Dr. Takaiwa, Dr. De Lumen will attempt to clone and sequence a cDNA encoding a nuclear protein that binds to the 5' upstream region of a rice glutelin gene, then to establish the stage-specific expression of this cDNA, and finally to compare its homology with cloned transcription factors. This project depends heavily on the groundwork established by Dr. Takaiwa in identifying upstream DNA control sequences in a rice glutelin gene that interact with nuclear proteins. This work should facilitate the cloning of similar transcription factors using methionine-rich protein genes from soybeans when Dr. De Lumen returns to his laboratory in Berkeley. This award is funded under the auspices of the Center for Global Partnership (CGP) of the Japan Foundation under the NSF-CGP Science Fellowship Program. ***