NSF-CGP Science Fellowship Program: Gene Targeting of RBCS18in Arabidopsis thaliana

该提案支持加州大学伯克利分校植物生物系博士后John Jelesko博士与日本日立高级研究实验室（HARL）的Masaki Furuya博士的合作，以开发一种易于处理的方法来产生植物中的特定基因靶向（基因敲除）事件。项目资金将允许耶列斯科博士在古屋博士的实验室参与为期六个月的研究合作。在此期间，Jelesko博士将使用单光子计数视频成像设备测量转基因植物体内荧光素酶的活性，这些植物经历了特定的基因靶向事件。基因靶向技术的成功开发将为Jelesko博士和Furuya博士提供一种强大的基因技术，以解剖高度同源的植物多基因家族的个体成员的作用。该提案汇集了美国和日本在植物分子遗传学领域工作的研究科学家。这位美国科学家将有机会熟悉哈佛大学独特的研究技术、设备和设施，并为古屋博士的整体研究工作做出贡献。因此，该提案实现了该计划通过国际合作交流和转移科学知识的目标。＊＊＊

9622319 Jelesko This proposal supports the collaboration of Dr. John Jelesko, a Postdoctoral Fellow at the University of California Berkeley Plant Biology Department, with Dr. Masaki Furuya, at the Hitachi Advanced Research Laboratory (HARL) in Japan, in order to develop a tractable approach to generate specific gene targeting (gene knockout) events in plants. Project funding will allow Dr. Jelesko to participate in a six month research collaboration in Dr. Furuya's laboratory. During this time, Dr. Jelesko will use single photon counting video imaging equipment to measure in vivo luciferase activity in transgenic plants which have undergone a specific gene targeting event. Successful development of a gene targeting technology will provide Dr. Jelesko and Dr. Furuya with a powerful genetic technique to dissect the role of individual members of highly homologous plant multigene families. This proposal brings together research scientists from the USA and Japan working in the area of plant molecular genetics. The US scientist will have an opportunity to become familiar with research techniques, equipment, and facilities unique to HARL as well as contributing to the overall research efforts of Dr. Furuya. Therefore, this proposal fulfills the objectives of the Program in its exchange and transfer of scientific knowledge through international collaboration. ***