NSF-CGP Science Fellowship Program: Gene Targeting of RBCS18in Arabidopsis thaliana

9622319 Jelesko这项建议支持加州大学伯克利分校植物生物学系博士后John Jelesko博士与日本日立高级研究实验室(HARL)的古屋正明博士的合作，以开发一种在植物中产生特定基因靶向(基因敲除)事件的简便方法。项目资金将允许杰莱斯科博士在古屋博士的实验室参与为期六个月的研究合作。在此期间，Jelesko博士将使用单光子计数视频成像设备来测量经历了特定基因靶向事件的转基因植物的体内荧光素酶活性。基因打靶技术的成功开发将为杰莱斯科博士和古屋博士提供一种强大的基因技术，来剖析高度同源的植物多基因家族中单个成员的作用。这项提议汇集了美国和日本在植物分子遗传学领域工作的研究科学家。这位美国科学家将有机会熟悉哈尔独有的研究技术、设备和设施，并为古屋博士的整体研究努力做出贡献。因此，这项建议实现了该计划通过国际合作交流和转让科学知识的目标。***

9622319 Jelesko This proposal supports the collaboration of Dr. John Jelesko, a Postdoctoral Fellow at the University of California Berkeley Plant Biology Department, with Dr. Masaki Furuya, at the Hitachi Advanced Research Laboratory (HARL) in Japan, in order to develop a tractable approach to generate specific gene targeting (gene knockout) events in plants. Project funding will allow Dr. Jelesko to participate in a six month research collaboration in Dr. Furuya's laboratory. During this time, Dr. Jelesko will use single photon counting video imaging equipment to measure in vivo luciferase activity in transgenic plants which have undergone a specific gene targeting event. Successful development of a gene targeting technology will provide Dr. Jelesko and Dr. Furuya with a powerful genetic technique to dissect the role of individual members of highly homologous plant multigene families. This proposal brings together research scientists from the USA and Japan working in the area of plant molecular genetics. The US scientist will have an opportunity to become familiar with research techniques, equipment, and facilities unique to HARL as well as contributing to the overall research efforts of Dr. Furuya. Therefore, this proposal fulfills the objectives of the Program in its exchange and transfer of scientific knowledge through international collaboration. ***