RUI: Determination of the Dorsal-Ventral Neural Axis in Xenopus

RUI：非洲爪蟾背腹神经轴的测定

• 批准号: 9406326
• 负责人: Margaret Saha
• 金额: $ 25.48万
• 依托单位: College of William and Mary
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9406326&HistoricalAwards=false
• 关键词: RUI; Determination; Dorsal; Ventral; Neural

The diversity of neuronal cell types in vertebrates derives from regional differences along the anterior-posterior (A/P) as well as the dorsal-ventral (D/V) axes of the developing nervous system. Previous work has shown that A/P properties are extremely labile during the initial phases of regionalization. In order to investigate the corresponding stages of neural regionalization along the D/V axis, this investigator initiated a series of experiments examining the early phases of D/V neural patterning using the amphibian Xenopus as a model system. Preliminary data suggest a model in which D/V pattern in the prospective spinal cord region is fixed at relatively early stages in contrast to the anterior brain region in which D/V pattern remains labile for a prolonged period of time. Using tissue manipulations in conjunction with molecular approaches, this proposal will test and extend this model by systematically examining the tissue interactions governing the early stages of D/V neural patterning. These experiments will not only provide a framework for linking gene expression with biological processes mediating D/V patterning events but will also contribute to an understanding of the unique properties of embryonic neuronal tissue, particularly its enormous plasticity.

脊椎动物神经元细胞类型的多样性来源于发育神经系统的前后（A/P）以及背腹（D/V）轴的区域差异。 以前的工作表明，A/P属性是非常不稳定的区域化的初始阶段。 为了研究神经区域化沿着的D/V轴的相应阶段，本研究员开始了一系列的实验研究的早期阶段的D/V神经图案作为一个模型系统的两栖动物爪蟾。 初步的数据表明，在该模型中，在未来的脊髓区域的D/V模式是固定在相对较早的阶段，在大脑前部区域，其中D/V模式保持不稳定的一段较长的时间。 使用组织操作与分子方法相结合，该建议将测试和扩展这个模型，系统地检查组织的相互作用，D/V神经模式的早期阶段。 这些实验不仅将提供一个框架，基因表达与介导D/V图案化事件的生物过程，但也将有助于了解胚胎神经元组织的独特性质，特别是其巨大的可塑性。