De Novo Protein Synthesis: Design and Assembly of Uniquely Folded Supersecondary Structural Motifs

9412442 Imperiali本研究的重点是组装长度为20-40个氨基酸的“紧凑”超二级结构基序，用于从头合成蛋白质。 特异性靶标包括三链反平行β折叠基序，其在结构上基于从α-神经毒素的同源家族切除的结构域，以及分别在蝎毒素和锌指蛋白之后图案化的α、β、β和β、α、α基序。 这些研究将评估使用定制的基序制备模板的潜力，这些模板反过来又可用于催化剂的构建。 %有了这个更新的奖项，合成有机计划正在支持加州理工学院化学与化学工程系的Barbara Imperiali博士的研究。 Imperiali教授将把她的工作重点放在理解和操纵多肽结构上。 这项工作将涉及组装结构上确定的多肽基序，可以用作多功能的三维模板，用于构建催化活性实体。

9412442 Imperiali The focus of this research is the assembly of "compact" supersecondary structural motifs of 20-40 amino acids in length for the de novo synthesis of proteins. Specific targets include a triple-stranded antiparallel beta-sheet motif, structurally based on a domain excised from the homologous family of alpha-neurotoxins, and alpha,beta,beta and beta, alpha,alpha motifs patterned after the scorpion toxins and zinc finger proteins respectively. The studies will evaluate the potential for using tailored motifs for the preparation of templates, useful in turn, for the costruction of catalysts. %%% With this renewal award, the Synthetic Organic Program is supporting the research of Dr. Barbara Imperiali of the Division of Chemistry and Chemical Engineering at the California Institute of Technology. Professor Imperiali will focus her work on understanding and manipulating polypeptide architecture. The work will involve the assembly of structurally defined polypeptide motifs that can be used as versatile three dimensional templates for the construction of catalytically active entities.