Design and Oligomerization of Uniquely Folded Supersecondary Structural Motifs

独特折叠超二级结构基序的设计和寡聚化

• 批准号: 0100735
• 负责人: Barbara Imperiali
• 金额: --
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0100735&HistoricalAwards=false
• 关键词: Design; Oligomerization; Uniquely; Folded; Supersecondary

With the support of the Organic and Macromolecular Chemistry Program, Professor Barbara Imperiali, of the Department of Chemistry at the Massachusetts Institute of Technology, is studying the structure, association properties, and function of "mini-protein" motifs. A 29-residue mini-motif containing a disulfide link will be rationally designed to a disulfide-free motif, and a biological strategy for "evolving" 23- and 29-residue motifs will be used to develop mini-motifs including only the 20 encoded amino acids, rather than relying on one or two D-amino acids currently used as turn stabilizing agents. New methods, including the use of fluorescent reporter groups and amino acids with solvatochromic properties, will be implemented to permit the discovery of peptide oligomers with discrete quaternary structure. Finally, Professor Imperiali will explore the possible function of these mini-motifs in the molecular recognition and sensing of small molecules such as fluorescent organic species and fluorescently labeled mono- and disaccharides.Proteins (polypeptides), comprised of long chains of interconnected amino acids, play myriad biochemical roles. Their specific function is critically dependent on the three dimensional structure adopted by the polypeptide chain, yet the factors responsible for protein folding are still at best incompletely understood. Through the design, synthesis, and study of small polypeptides, Professor Barbara Imperiali, of the Department of Chemistry at the Massachusetts Institute of Technology, with the support of the Organic and Macromolecular Chemistry Program, is shedding light on the factors responsible for the formation of particular protein folding motifs. Her studies also explore the possibility that these "mini-motifs" could serve as scaffolds to permit the recognition and sensing of small organic molecules, including sugars, leading in the longer term to the design of peptide-based chemosensing agents.

在有机和大分子化学项目的支持下，麻省理工学院化学系的Barbara Imperiali教授正在研究“迷你蛋白质”基序的结构、结合特性和功能。含有二硫化物链接的29个残基的迷你基序将被合理地设计成不含二硫化物的基序，而“进化”23和29个残基的生物学策略将被用来开发只包含20个编码氨基酸的迷你基序，而不是依赖于目前作为稳定剂的一两个d -氨基酸。新的方法，包括使用荧光报告基团和具有溶剂变色性质的氨基酸，将被实施，以允许发现具有离散的四级结构的肽低聚物。最后，Imperiali教授将探讨这些微型基元在小分子（如荧光有机物种和荧光标记的单糖和双糖）的分子识别和传感中的可能功能。蛋白质（多肽）由相互连接的氨基酸长链组成，发挥着无数的生化作用。它们的特定功能严重依赖于多肽链所采用的三维结构，但负责蛋白质折叠的因素充其量仍未完全了解。通过小多肽的设计、合成和研究，麻省理工学院化学系的Barbara Imperiali教授在有机和大分子化学项目的支持下，正在揭示导致特定蛋白质折叠基元形成的因素。她的研究还探索了这些“微型基序”作为识别和感知小有机分子（包括糖）的支架的可能性，从长远来看，这将导致基于肽的化学感知剂的设计。