Acquisition of equipment for shared embryonic stem-cell and transgenic animal facility

购置共享胚胎干细胞和转基因动物设施的设备

• 批准号: 9413521
• 负责人: Kimi Hatton
• 金额: $ 10.56万
• 依托单位: Temple University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-10-01 至 1996-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9413521&HistoricalAwards=false
• 关键词: Acquisition; equipment; shared; embryonic; stem

ABSTRACT In this project, we request (1) one high-quality microscope with phase contrast and DIC optics; (2) two vibration free air tables with one base plate, (3) two rnicromanipulators, (4) one dissecting microscope, (5) one computer, (6) one cage- washer, (7) one CO2 incubator, (8) one biosafety cabinet and one laminar flow hood, and (8) caging and racks. The requested equipment is focused on the central theme of normal mouse development, genetics and molecular biology and will facilitate and provide for research, research services and research training for undergraduates, graduates, postdoctoral students and investigators. The equipment will enable maintenance of state- of-the-art research initiatives in cutting-edge research and accommodate newly recruited faculty at Temple in the Fels Research Institute. The specialized equipment is a requirement for our program in Developmental Biology. To maintain our position at the frontier of research, we have developed five well- defined basic science programs (1) Molecular Biology and Oncology program, (2) Immunology program (3) Biochemistry program (4) Developmental Biology Program, and (5) Molecular Pharmacology/ Chemical Carcinogenesis program. The Fels Institute brings together at one location a diversity of intellectual skills and research experience. Our goal is to address questions of critical importance in each program, particularly in the area of Developmental Biology using the most powerful methods of analysis currently available. Over the last year, we have actively recruited a number of new investigators to Fels. Acquisition of the requested equipment will help fulfill a major research objective of our Program in that we will effectively address questions concerning the normal role of a particular gene during development and the basic biology and regulation of gene sequences during development. This equipment will help to bridge the gap between an investigator's ability to define the r ole of a gene in simple tissue culture systems and in the organism. In this proposal, we ask for a related series of equipment dedicated for the purpose of conducting gene-targeting and transgenic experiments in our basic Developmental Biology Program. The equipment will be placed in a pathogen- free Barrier animal facility of Temple University. This facility is a shower-in facility staffed by two animal technicians, one manager and one Veterinarian. Experiments are conducted in the facility and common-use equipment is located there for all investigators using the barrier. The area is currently providing IACUUC and AALAC approved housing and care of animals used in research and research training. In a separate initiative, we are planning repair and renovation of this space; however, the proposed series of instruments will function for the same purpose whether or not building renovations occur. In this project, the acquisition of this equipment will allow us to perform blastocyst and egg injections at the same time, will allow efficient functioning of the animal facility, and will allow participation of more research laboratories in Developmental biology research and research training.

摘要 在这个项目中，我们要求（1）一个高品质的显微镜与相位衬度和DIC光学;（2）两个带有一个底板的无振动空气台，（3）两个显微操作器，（4）一个解剖显微镜，（5）一台计算机，（6）一个洗笼机，（7）一个CO2培养箱，（8）一个生物安全柜和一个层流罩，（8）笼架。所要求的设备侧重于小鼠正常发育、遗传学和分子生物学这一中心主题，并将便利本科生、研究生、博士后学生和调查人员的研究、研究服务和研究培训。这些设备将使最先进的研究举措在尖端研究的维护和适应新招聘的教师在寺在费尔斯研究所。专门的设备是我们在发育生物学计划的要求。为了保持我们在研究前沿的地位，我们开发了五个定义明确的基础科学项目（1）分子生物学和肿瘤学项目，（2）免疫学项目，（3）生物化学项目，（4）发育生物学项目，和（5）分子药理学/化学致癌项目。费尔斯研究所汇集了在一个位置的智力技能和研究经验的多样性。 我们的目标是解决每个程序中至关重要的问题，特别是在发育生物学领域，使用目前最强大的分析方法。在过去的一年里，我们积极招募了一些新的调查员到费尔斯。获得所要求的设备将有助于实现我们计划的主要研究目标，因为我们将有效地解决有关特定基因在发育过程中的正常作用以及发育过程中基因序列的基础生物学和调控的问题。这种设备将有助于弥补研究人员在简单的组织培养系统和生物体中确定基因作用的能力之间的差距。 在本提案中，我们要求提供一系列相关设备，专门用于在我们的基本发育生物学计划中进行基因靶向和转基因实验。该设备将被放置在坦普尔大学的无病原体屏障动物设施中。该机构是一个临时机构，由两名动物技术员、一名经理和一名兽医组成。实验在该设施中进行，并为使用屏障的所有调查人员提供了通用设备。该地区目前正在提供IACUUC和AALAC批准的住房和护理用于研究和研究培训的动物。在一项单独的倡议中，我们正在计划对这一空间进行维修和翻新;然而，无论是否进行建筑翻新，拟议的一系列仪器都将发挥同样的作用。 在这个项目中，购买这种设备将使我们能够同时进行囊胚和卵子注射，使动物设施能够有效运作，并使更多的研究实验室参与发育生物学研究和研究培训。