Purchase of Equipment for Integrated Research on Plant Responses to Environmental Stress

摘要 该项目寻求资金购买仪器，专门用于“植物对环境压力的反应”领域的研究。 所要求的资金不仅将为我们的“植物胁迫”研究计划提供现代仪器，而且还将加强我们在植物胁迫生理学最现代技术方面培训研究生和本科生的能力。 在本建议范围内进行的研究重点比较广泛，因为它将包括几个不同的研究项目。 然而，该项目将加强的所有研究项目都属于植物生理学和代谢对环境胁迫的反应的一般类别。 其中一个项目将使用所要求的仪器中心 上 发展 高粱抗旱基因型选育的生理选择标准。 这一持续的国际研究计划与墨西哥已得到联邦和国家的支持，已发展成为一个富有成效的合作，现在涉及几个研究人员和学生都迈阿密大学和塔毛利帕斯州自治大学在墨西哥东北部。 在第二 研究计划 的 将 使用 的 我们正在研究植物表皮类黄酮作为W-B响应分子的作用和W-B保护机制。 我们已经合成了（世界上只有两个实验室做这件事），一种植物酚类化合物的特异性抑制剂，能够完全抑制类黄酮的合成。 这种抑制剂独特地使我们能够测试的假设，表皮类黄酮是重要的W-B屏幕在植物中。

ABSTRACT This project seeks funds to purchase instrumentation that will be used to conduct research specifically in the area of "plant responses to environmental stress". The funds requested will not only provide our "plant stress" research program with modern instrumentation, but will also strengthen our capabilities for training graduate and undergraduate students in the most contemporary techniques in plant stress physiology. The focus of the research to be conducted within the scope of this proposal is somewhat broad in the sense that it will encompass several different research projects. However, all of the research projects that will be enhanced by this project fall into the general category of plant physiology and metabolism as a response to environmental stress. One of the projects that will use the requested instrumentation centers on the development of physiological selection criteria for breeding drought resistant genotypes of grain sorghum. This continuing international research program with Mexico which has received federal and state support, has developed into a fruitful collaboration which is now involving several researchers and students from both Miami University and the Autonomous University of Tamaulipas in northeastern Mexico. In a second research program that will use the requested instrumentation, we are studying the role of epidermal flavonoids as W-B response molecules and W-B protection mechanisms in plants. We have synthesized (only one of two labs in the world to do this), a specific inhibitor of plant phenolics that is capable of completely inhibiting the synthesis of flavonoids. This inhibitor has uniquely enabled us to test the hypothesis that epidermal flavonoids are important W-B screens in plants.