Development of a Near-Field Scanning Optical Microscope Capable of Detection and Resolution of Individual Fluorescent Molecules

开发能够检测和分辨率单个荧光分子的近场扫描光学显微镜

• 批准号: 9415294
• 负责人: Sheldon Schultz
• 金额: $ 4.67万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-15 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9415294&HistoricalAwards=false
• 关键词: Development; Near; Field; Scanning; Optical

We have recently demonstrated that a new form of near field scanning optical microscopy based on a small (e.g. 35 nm diameter)Ag sphere, excited at its plasmon resonant frequency, can achieve sub-wavelength magnetic Kerr imaging. We propose to develop a suitably modified microscope based on these Ag plasmon resonators, but specifically optimized for high resolution fluorescence imaging. We believe that the detection of individual fluorophores with near molecular resolution may be possible. Example are given of exciting new opportunities in bio-medical research that will become practicable when our new microscope is operative.

我们最近证明了一种新的形式的近场扫描光学显微镜，它基于一个小的(例如，直径35 nm)的银球，以其等离子体激元的共振频率激发，可以实现亚波长的磁克尔成像。我们建议开发一种基于这些银等离子激元谐振器的适当改进的显微镜，但专门针对高分辨率荧光成像进行优化。我们相信，以接近分子分辨率的方式检测单个荧光团是可能的。举例说明了生物医学研究中令人振奋的新机会，当我们的新显微镜投入使用时，这些机会将变得可行。