Designing Effective Immunotoxins: The Relationship Between Cellular Processing and Cytotoxicity

点击翻译按钮获取中文摘要

9417006 Murphy This project is on research to characterize and model the response of tumor cells to targeted toxins, and to develop design strategies for improving the efficacy of targeted toxins. This research has two objectives: (1) the cellular processing kinetics of several combinations of target, targeting agent, and cell lines are to be experimentally determined and used to develop mathematical models, which are to relate cellular trafficking of the target agent to protein synthesis inhibition, and describe the time these molecules spend inside the cell; and (2) the feasibility will be explored of improving the efficacy of targeted toxins by chemical modifications of the targeting agent in order to increase the time the targeting agent is to reside in the Golgi apparatus. This research could contribute to rational drug design while generating new knowledge on immunotoxins. ***