Analysis of the Inverse Effect in Drosophila

果蝇逆效应分析

• 批准号: 9417814
• 负责人: James Birchler
• 金额: $ 39.8万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9417814&HistoricalAwards=false
• 关键词: Analysis; Inverse; Effect; Drosophila

9417814 Birchler The white locus of Drosophila melanogaster will be used as a target gene to study the action of trans-acting dosage modifiers and their potential involvement with coordinate gene expression, dosage compensation, aneuploid syndromes, quantitative traits and position effect variegation. As an approach to the ultimate goal, examples of dosage sensitive regulators will be cloned and characterized, interactions between such dosage sensitive regulators and the male specific lethal locus characterized and a search for recessive mutations which modify the white gene will be made. %%% The regulation of gene expression in multicellular organisms is not a simple linear mechanism. A number of different mechanisms have been or are being described . Over the past several years a regulatory network that results in an inverse dosage effect on the expression of the genes which they affect has been analyzed by this laboratory and this analysis will be continued. ***

9417814 Birchler黑腹果蝇的白色基因座将被用作靶基因，研究反式作用剂量调节剂的作用及其与协调基因表达、剂量补偿、非整倍体综合征、数量性状和位置效应杂色的潜在参与。作为达到最终目标的方法，将克隆和表征剂量敏感调节剂的实例，表征这种剂量敏感调节剂与雄性特异性致死基因座之间的相互作用，并将进行修饰白色基因的隐性突变的搜索。 多细胞生物中基因表达的调节不是一个简单的线性机制。已经或正在描述一些不同的机制。在过去几年中，本实验室分析了一个调节网络，该网络对受其影响的基因的表达产生了反向剂量效应，并将继续进行这一分析。 ***