CORE--DNA SEQUENCING FACILITY

核心——DNA测序设备

• 批准号: 7142493
• 负责人: Robert C Young
• 金额: $ 10.14万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142493
• 关键词: DNA; biomedical facility; genetic polymorphism; high throughput technology; neoplasm /cancer; nucleic acid sequence

The Automated DNA Sequencing Facility is a merged Facility which provides investigators with state-of-the-art DNA sequencing and analysis of DNA polymorphisms. The Facility is used by 52 peer-reviewed funded investigators across 10 Research Programs from all three Divisions. Ninety-nine and one-half percent (99.5%) of the users have peer-reviewed funding. Richard Hardy, Ph.D. (Program Leader, Immunobiology) assumed direction of the Facility in 1993. Automated sequencing using an ABI Model 373 was set up in 1994. Model 377 sequencers were subsequently added, one in 1995, and another in 1997. A third 377 replaced the original 373 in 2002. Recently, two of the 377s have been replaced by a modern ABI 3100 capillary instrument, providing more reliable high-throughput sequencing. The Sequencing Facility provides Fox Chase Cancer Center (FCCC) investigators with computer-readable sequences of their DNA samples in a timely and cost effective manner, generating data more rapidly and of greater read-length compared to manual sequencing. Automated sequence analysis is used by investigators to verify DNA constructs, to identify mutations, and to determine the structure of newly cloned genes. Sequencing large genes (5-20 Kb) is much more tractable using automated sequence analysis, generating results in a few weeks, as compared to months using manual sequencing. The volume of usage of the Facility has continued to increase steadily over the past five years from 17,000 sequences in 1998 to over 28,000 in 2003 (64% increase). In addition to sequencing, analysis of DNA polymorphisms, important in genetic mapping studies, is also available in the Facility using "GeneScan" software on the 377. Equitable access to the Facility is assured by a Faculty Oversight Committee. The Facility was rated "Outstanding" at the last CCSG review.

自动DNA测序设施是一个合并的设施，为研究人员提供 最先进的DNA测序和DNA多态性分析。该设施由52个 来自所有三个部门的10个研究项目的同行评审资助研究人员。百分之九十九点五（99.5%）的用户有同行评审的资金。Richard哈代博士（免疫生物学项目负责人）于1993年担任该机构的领导。1994年建立了使用ABI Model 373的自动测序。随后又增加了377型测序仪，一个在1995年，另一个在1997年。2002年，第三批377辆取代了最初的373辆。最近，其中两台377已被现代ABI 3100毛细管仪器取代，提供更可靠的高通量测序。测序设施以及时和具有成本效益的方式为Fox Chase癌症中心（FCCC）的研究人员提供其DNA样本的计算机可读序列，与手动测序相比，更快地生成数据并具有更大的读取长度。自动序列分析被研究人员用来验证DNA结构，识别突变，并确定新克隆基因的结构。与使用手动测序的数月相比，使用自动化序列分析对大基因（5-20 Kb）进行测序更容易处理，在几周内产生结果。在过去五年中，该设施的使用量持续稳步增长，从1998年的17，000个序列增加到2003年的28，000多个序列（增长64%）。除了测序外，该设施还利用377型计算机上的“GeneScan”软件进行DNA多态性分析，这对遗传图谱研究很重要。公平获得 该设施是由一个学院监督委员会保证。该设施被评为“杰出”， CCSG的最新评论