Analytical and Preparative Biochemical Equipment

分析和制备生化设备

• 批准号: 9419627
• 负责人: John Lowenstein
• 金额: $ 5.46万
• 依托单位: Brandeis University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-15 至 1997-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9419627&HistoricalAwards=false
• 关键词: Analytical; Preparative; Biochemical; Equipment

The objective of this application is to replace inoperative equipment and to upgrade obsolescent equipment of the shared instrumentation facility of the department. Each of the investigators on this application is involved in the preparation of pure proteins and nucleic acids and the study of their properties, structure, and function. These purifications require both low and high speed centrifugations. Most also involve the separation of complex mixtures of biomolecules into pure constituents. Recent technological innovations have made such separations much faster and simpler. To this end, we are requesting a perfusion chromatography apparatus with suitable detectors. Many of the activity measurements and most of the sequencing reactions involve radioactive assays. To this end, we are requesting a scintillation counter. All instruments will be used on a shared basis. Experience shows this to be the most cost-effective way to use modern instrumentation. Individual users will contribute to the maintenance contracts of these instruments once the warranties expire. The sharing of maintenance contracts is also the most cost-effective way of using the equipment.

该应用程序的目的是更换不起作用的设备并升级该部门共享仪器设施的过时设备。该应用程序的每位研究人员都参与纯蛋白质和核酸的制备以及它们的性质、结构和功能的研究。这些纯化需要低速和高速离心。大多数还涉及将复杂的生物分子混合物分离成纯净的成分。最近的技术创新使这种分离变得更快、更简单。为此，我们需要配备合适检测器的灌注色谱仪。许多活性测量和大多数测序反应都涉及放射性测定。为此，我们需要一个闪烁计数器。 所有仪器将在共享的基础上使用。经验表明这是使用现代仪器最经济有效的方式。 一旦保修期满，个人用户将签署这些仪器的维护合同。共享维护合同也是使用设备最具成本效益的方式。