Purchase of a preparative HPLC system for bioactive peptides research

购买制备型 HPLC 系统用于生物活性肽研究

• 批准号: RTI-2018-00531
• 负责人: Aluko, Rotimi
• 金额: $ 6.86万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642198
• 关键词: Purchase; preparative; HPLC; system; bioactive

Food protein-derived peptides remain a critical source of information for the discovery of fundamental scientific knowledge on relationships between the structure and function of bioactive agents. This is because of the almost non-exhaustive possible combinations of the 20 naturally-occurring amino acids within the context of peptide chain length (2-20 residues). However, due to the similarity in peptide size and chain length, the isolation, purification, functional characterization, and in vivo testing of bioactive peptides require quantitative analysis using high pressure liquid chromatography (HPLC) systems. At the Department of Food and Human Nutritional Sciences, University of Manitoba, the 10-yr old preparative HPLC (Prep-HPLC) system available for my NSERC peptide Discovery research will no longer be serviceable after July 2018. This is because the manufacturer has discontinued the model and given us notice that manufacture of critical parts needed for regular operation has been discontinued. Taking into consideration the instrument's age, it is normal for us to perform critical maintenance and parts replacements every 6-8 months but this will no longer be available by August 2018. Thus, a breakdown of the instrument will effectively end its use and leave my research program without a system suitable for bio-assay guided quantitative purification of peptides, which is the cornerstone of my NSERC Discovery research program. There is no other Prep-HPLC system available at the Fort Garry campus or the medical campus of the University of Manitoba. Therefore, the aim of this proposal is purchase a new Prep-HPLC system that will enable my Discovery program to continue innovative characterization of the structure-function properties of food protein-derived peptides. The Prep-HPLC provides the most rapid and cost-effective means of separating and purifying biological molecules in quantities that are large enough to permit testing in multiple in vitro assays and animal disease models. This is because chronic animal feeding experiments are the most suitable for determining the ability of bioactive compounds to act on a long-term basis in preventing or reducing the pathological intensity of diseases. The Prep-HPLC is also critical to training of Highly Qualified Personnel that includes one postdoctoral fellow and 6 graduate students because it enables provision of fractions in quantities that are required for high quality thesis work and timely graduation. Technology developed with the instrument will facilitate value-added utilization of Canadian-grown crops or livestock with significant returns to the Canadian economy. The instrument will be used to facilitate interdisciplinary training of graduate students, postdoctoral fellows and undergraduate students in the areas of food science and life sciences related to preventive bioactive agents for homeostasis maintenance.

食物蛋白源肽仍然是发现生物活性剂的结构和功能之间关系的基础科学知识的重要信息来源。这是因为在肽链长度（2-20个残基）的背景下，20种天然存在的氨基酸的几乎非穷尽的可能组合。然而，由于肽大小和链长的相似性，生物活性肽的分离、纯化、功能表征和体内测试需要使用高压液相色谱（HPLC）系统进行定量分析。在马尼托巴大学食品与人类营养科学系，我的NSERC肽发现研究可用的10年前的制备型HPLC（Prep-HPLC）系统将在2018年7月后不再可用。这是因为制造商已经停止了该型号，并通知我们，正常运行所需的关键部件的制造已经停止。考虑到仪器的使用年限，我们通常每6-8个月进行一次关键维护和部件更换，但到2018年8月将不再提供。因此，该仪器的故障将有效地终止其使用，并使我的研究计划没有适合于生物测定指导的肽定量纯化的系统，这是我的NSERC发现研究计划的基石。在Fort Garry校区或马尼托巴大学医学校区没有其他Prep-HPLC系统。因此，本提案的目的是购买一个新的Prep-HPLC系统，这将使我的Discovery计划能够继续对食品蛋白衍生肽的结构-功能特性进行创新表征。Prep-HPLC提供了分离和纯化生物分子的最快速和最具成本效益的方法，这些生物分子的数量足够大，可以在多种体外试验和动物疾病模型中进行测试。这是因为慢性动物喂养实验最适合于确定生物活性化合物在预防或降低疾病的病理强度方面长期起作用的能力。制备型HPLC对培养高素质人才也至关重要，其中包括一名博士后研究员和6名研究生，因为它能够提供高质量论文工作和及时毕业所需的分数。利用该工具开发的技术将促进加拿大种植的作物或牲畜的增值利用，为加拿大经济带来重大回报。该工具将用于促进对研究生、博士后研究员和本科生进行与维持体内平衡的预防性生物活性剂有关的食品科学和生命科学领域的跨学科培训。