Responses of Antarctic Phytoplankton to Small-Scale Changes in Temperature

过去的报告表明，南极浮游植物对微小的热变化极为敏感。然而，由于与当前可用方法相关的问题，实际的文档编制是困难的。与温度变化相关的细胞死亡和/或代谢停滞的记录可能会影响我们对南极生态的理解，而与海冰形成和退缩相关的记录则较少。本项目探讨了一些新开发的荧光探针在生物医学研究中的潜在应用，以评估哺乳动物细胞生物学到生物海洋学，即浮游植物代谢活性和细胞死亡。然而，荧光探针对于评估浮游植物细胞在温度胁迫下的生理状态可能有用，也可能没有用处。如果制定出适当的方案，生物海洋学家将能够检查和评估极地浮游植物对极地海冰形成和消退的温度反应。

9420085 Shapiro Past reports suggest that Antarctic phytoplankton are hypersensitive to small thermal changes. However, actual documentation is difficult because of problems associated with currently available methods. Documentation of cell death and/or metabolic arrest, associated with changes in temperature less than that associated with formation and retreat of sea ice, could affect our understanding of Antarctic ecology. This project investigates the potential application of some recently developed fluorescent probes in biomedical research for evaluating mammalian cell biology to biological oceanography, i.e., phytoplankton metabolic activity and cell death. However, fluorescent probes may or may not prove useful for evaluating the physiological state of phytoplankton cells in response to temperature stress. If appropriate protocols are worked out, biological oceanographers will be able to examine and evaluate temperature responses of polar phytoplankton in response to formation and retreat of polar sea ice.