SGER: The Role of Metal Reduction in the Relationship Between Marine Phytoplankton and Their Associated Bacteria

SGER：金属还原在海洋浮游植物及其相关细菌之间关系中的作用

• 批准号: 9614005
• 负责人: Lynda Shapiro
• 金额: $ 4.97万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1998-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9614005&HistoricalAwards=false
• 关键词: SGER; Role; Metal; Reduction; Relationship

9614005 SHAPIRO In the relationship between phytoplankton and their associated bacteria, it is assumed that the bacteria derive nutrition from phytoplankton exudates, and that phytoplankton utilize the bacteria as a source of specific vitamins, a source of N or P, or as an alternate carbon source. This SGER project hypothesizes that a major role of this association relates to metal reduction and acquisition. Thus, the phycosphere will be examined as a symbiotic zone of active metal reduction by bacteria and uptake by phytoplankton. As a first step in examining this hypothesis, the researchers will determine whether there are replicable assemblages of bacteria living in close association with phytoplankters, and whether these associations are capable of metal reduction. During this SGER project phytoplankton clones from the Oregon coast will be established and will subsequently be tested with 16s rRNA probe analysis for common marine bacterial genera, including Shewanella putrefaciens, a common metal-reducing species. Population analysis of bacteria associated with phytoplankton natural samples, using DNA amplification, will permit the researchers to characterize bacteria associated with phytoplankton whether or not they are amenable to culturing techniques. Natural samples will be probed with 16s rRNA probes generated from the amplified DNA. The significance of this research lies in its potential to provide a mechanism, based on symbiosis, for metal uptake by phytoplankton. This may prove critical in understanding those regions of the world's oceans where metal limitation of primary production is strongly suggested. ***

小行星9614005 在浮游植物与其伴生细菌之间的关系中，假设细菌从浮游植物分泌物中获得营养，浮游植物利用细菌作为特定维生素的来源，N或P的来源，或作为替代碳源。 这个SGER项目假设这种关联的主要作用与金属还原和获取有关。因此，藻圈将被视为细菌还原活性金属和浮游植物吸收活性金属的共生区。 作为检验这一假设的第一步，研究人员将确定是否存在与植物共生菌密切相关的可复制细菌组合，以及这些组合是否能够进行金属还原。 在这个SGER项目中，将建立来自俄勒冈州海岸的浮游植物克隆，随后将对常见的海洋细菌属进行16 s rRNA探针分析，包括希瓦氏腐败菌（Shewanella putrefaciens），一种常见的金属还原物种。 人口 利用DNA扩增技术对浮游植物天然样本中的细菌进行分析，将使研究人员能够确定浮游植物中细菌的特征，无论它们是否适合培养技术。 天然样品将用扩增DNA生成的16s rRNA探针进行探测。 这项研究的意义在于它的潜力，提供了一种机制，共生的基础上，浮游植物的金属吸收。 这可能对了解强烈建议限制初级生产金属的世界海洋区域至关重要。 ***