Automated DNA Sequencer and Ancillary Equipment for a Phosphoimager

用于磷光成像仪的自动 DNA 测序仪和辅助设备

• 批准号: 9421031
• 负责人: Robert Haselkorn
• 金额: $ 6.5万
• 依托单位: University of Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-06-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9421031&HistoricalAwards=false
• 关键词: Automated; DNA; Sequencer; Ancillary; Equipment

9421031 Haselkorn This multi-user equipment award provides funds for an automated DNA sequencer and associated components. The principal users are members of the Department of Molecular Genetics and Cell Biology. The research themes of the users are united by interests in gene organization and regulation, with the organisms being studied including bacteria, yeast, and plants. The bacterial work concentrates on the photosynthetic Anabaena bacteria. More than 1,000 genes are being studied whose expression is differentially induced by the production of heterocysts. Yeast work is concentrating on regions of the chromosomes that are relatively silent in gene expression and the identification of other genes that can induce expression in the silent regions. The plant work is primarily on Arabidopsis, where genes required for interaction between pollen grains and the pistil during fertilization are being identified. Genes from other organisms that have similar DNA base sequences are being isolated and sequenced. A gene family that codes for the chloroplast processing enzyme is being identified. This family is responsible for enabling proteins to enter the chloroplasts, where photosynthesis occurs. Finally, genes from wheat that are involved in herbicide resistance are being sequenced in order to see how they differ from the genes in sensitive plants. %%% The sequencing being carried out with this machine is important for our understanding of bacterial development, gene expression in yeast, and mechanisms of herbicide resistance in crop plants. ***

9421031 Haselkorn 这项多用户设备奖为自动 DNA 测序仪和相关组件提供资金。主要用户是分子遗传学和细胞生物学系的成员。用户的研究主题因对基因组织和调控的兴趣而结合在一起，所研究的生物体包括细菌、酵母和植物。细菌的工作集中在光合鱼腥藻细菌上。超过 1,000 个基因正在研究中，这些基因的表达因异形细胞的产生而受到差异性诱导。酵母的工作集中在基因表达相对沉默的染色体区域，以及鉴定可以在沉默区域诱导表达的其他基因。植物工作主要针对拟南芥，正在鉴定受精过程中花粉粒和雌蕊之间相互作用所需的基因。来自其他生物体的具有相似 DNA 碱基序列的基因正在被分离和测序。编码叶绿体加工酶的基因家族正在被鉴定。该家族负责使蛋白质进入叶绿体，在那里发生光合作用。最后，正在对小麦中涉及除草剂抗性的基因进行测序，以了解它们与敏感植物中的基因有何不同。 %%% 使用该机器进行的测序对于我们了解细菌发育、酵母基因表达以及作物除草剂抗性机制非常重要。 ***