Automated DNA Sequencer and Ancillary Equipment for a Phosphoimager

该多用户设备奖为自动DNA测序仪和相关组件提供资金。主要用户是分子遗传学和细胞生物学部门的成员。用户的研究主题是基于对基因组织和调控的兴趣而统一的，所研究的生物包括细菌、酵母和植物。细菌的研究主要集中在光合作用的水藻细菌上。目前正在对1000多个基因进行研究，这些基因的表达是由异囊的产生引起的。酵母的工作主要集中在染色体中基因表达相对沉默的区域，以及鉴定可以诱导沉默区域表达的其他基因。植物方面的工作主要是在拟南芥上，在那里花粉粒和雌蕊在受精过程中相互作用所需的基因正在被确定。从其他具有相似DNA碱基序列的生物体中分离出的基因正在被测序。一个编码叶绿体加工酶的基因家族正在被发现。这个家族负责使蛋白质进入叶绿体，在那里进行光合作用。最后，正在对小麦中涉及抗除草剂的基因进行测序，以便观察它们与敏感植物中的基因有何不同。用这台机器进行的测序对我们了解细菌的发育、酵母的基因表达和作物抗除草剂的机制非常重要。＊＊＊

9421031 Haselkorn This multi-user equipment award provides funds for an automated DNA sequencer and associated components. The principal users are members of the Department of Molecular Genetics and Cell Biology. The research themes of the users are united by interests in gene organization and regulation, with the organisms being studied including bacteria, yeast, and plants. The bacterial work concentrates on the photosynthetic Anabaena bacteria. More than 1,000 genes are being studied whose expression is differentially induced by the production of heterocysts. Yeast work is concentrating on regions of the chromosomes that are relatively silent in gene expression and the identification of other genes that can induce expression in the silent regions. The plant work is primarily on Arabidopsis, where genes required for interaction between pollen grains and the pistil during fertilization are being identified. Genes from other organisms that have similar DNA base sequences are being isolated and sequenced. A gene family that codes for the chloroplast processing enzyme is being identified. This family is responsible for enabling proteins to enter the chloroplasts, where photosynthesis occurs. Finally, genes from wheat that are involved in herbicide resistance are being sequenced in order to see how they differ from the genes in sensitive plants. %%% The sequencing being carried out with this machine is important for our understanding of bacterial development, gene expression in yeast, and mechanisms of herbicide resistance in crop plants. ***