Light Regulation of Gene Expression in a Unicellular Cyanobacterium

蓝藻是一种进行绿色植物生长的原核生物 光合作用，在光线中释放氧气。 对于某些菌株， 存在一种基于DNA的方便的遗传分析系统， 介导转化 此外，蓝藻控制了 一些基因的表达，以响应数量的变化， 光的质量。 我们将利用基因百科全书（一个有序的 一组覆盖整个染色体的粘粒克隆） Synechocystis 6803定位响应光变化的基因 然后通过体外和体外分析， 调节这些基因所需的核苷酸序列（顺式作用 元素）和负责其差异的蛋白质 反式作用元件（trans-acting elements） 基因和 生物化学的方法应该可以让我们识别出 将光信号转导到基因中的调节级联 表情反应。

Cyanobacteria are prokaryotic organisms that carry out green plant photosynthesis, evolving oxygen in the light. For some strains, there exists a convenient system of genetic analysis based on DNA- mediated transformation. In addition, cyanobacteria control the expression of some genes in response to changes in the quantity or quality of light. We will exploit a gene encyclopedia (an ordered set of cosmid clones covering the entire chromosome) of Synechocystis 6803 to locate genes that respond to light changes and then to define, by in vitro and in vitro analyses, both the nucleotide sequences required to regulate those genes (cis-acting elements) and the proteins responsible for their differential expression (trans-acting elements). The combined genetic and biochemical approach should allow us to identify the components of the regulatory cascade that transduces a light signal into a gene expression response.