International Postdoctoral Fellows Program: Functional Analysis of the Coatomer Complex in the Secretory Pathway

9424028惠特尼国际青年研究员和博士后研究员计划使美国科学家和工程师能够在国外进行三到二十四个月的研究。该计划的奖励为联合研究提供了机会，并利用独特或互补的设施、专业知识和国外的实验条件。该奖项将支持詹姆斯·安德鲁·惠特尼博士与瑞士日内瓦大学托马斯·埃哈德·克瑞斯教授进行为期12个月的博士后研究访问。真核细胞的一个决定性特征是膜封闭的细胞器系统，新合成的分泌蛋白通过该系统传递到细胞表面。膜和分泌蛋白在这些细胞器之间的转移通常涉及不同转运囊泡群的形成。了解这些转运事件的分子机制已成为分子细胞生物学最重要的问题之一。本文提出的主要目标是了解更多有关分泌途径中囊泡选择性出芽的分子机制。特别是，PI将使用相对较新的体内成像技术来跟踪被认为在这一过程中起作用的细胞质蛋白的动态分布。＊＊＊

9424028 Whitney The International Junior Investigator and Postdoctoral Fellows Program enables U.S. scientists and engineers to conduct three to twenty-four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit by Dr. James Andrew Whitney to work with Professor Thomas Erhard Kreis at the University of Geneva in Switzerland. One of the defining features of eukaryotic cells is the system of membrane-enclosed organelles through which newly synthesized secretory proteins pass on their way to the cell surface. The transfer of membrane and secretory proteins between these organelles typically involves the formation of distinct populations of transport vesicles. Understanding the molecular mechanisms of these transport events has become one of the most important problems in molecular cell biology. The primary goal of the work proposed here is to learn more about the molecular mechanisms involved in the selective budding of vesicles in the secretory pathway. In particular, the PI will use relatively new in vivo imaging techniques to follow the dynamic distribution of cytosolic proteins thought to play a role in this process. ***