Advanced Undergraduate Laboratory Experiences in Modern Molecular Biotechnology

9452336霍普金斯实验室在分子和细胞生物学的经验是通过引入新的实验，并通过使现有的实验提供给更多的学生增强。这些经验有利于生物和化学专业以及科学教育和护理专业的学生。 为学生创造机会，以获得在研究，诊断和基因检测中使用的技术的实践经验。 学生使用发酵罐、高速离心机和均质器从微生物和真核细胞中大规模生产、分离和纯化DNA和蛋白质，并通过聚丙烯酰胺和琼脂糖凝胶电泳对这些分子进行表征。学生还将探索荧光显微镜和非放射性探针的Southern和Western印迹的分子探测技术。

9452336 Hopkins Laboratory experiences in molecular and cellular biology are enhanced by the introduction of new experiments and by making existing experiments available to larger numbers of students. These experiences benefit biology and chemistry majors as well as science education and nursing students. Opportunities are created for students to gain practical experience in techniques used in research, diagnostics, and genetic testing. Students undertake the large-scale production, isolation, and purification of DNA and proteins from microorganisms and eukaryotic cells by using a fermentor, high-speed centrifuge, and homogenizer, and they characterize these molecules by polyacrylamide and agarose gel electrophoresis. The students also explore technologies of molecular probing by fluorescence microscopy and Southern and Western blotting with nonradioactive probes.