Advanced Undergraduate Laboratory Experiences in Modern Molecular Biotechnology

现代分子生物技术的先进本科实验室经验

• 批准号: 9452336
• 负责人: Andrew Hopkins
• 金额: $ 3.08万
• 依托单位: Alverno College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9452336&HistoricalAwards=false
• 关键词: Advanced; Undergraduate; Laboratory; Experiences; Modern

9452336 Hopkins Laboratory experiences in molecular and cellular biology are enhanced by the introduction of new experiments and by making existing experiments available to larger numbers of students. These experiences benefit biology and chemistry majors as well as science education and nursing students. Opportunities are created for students to gain practical experience in techniques used in research, diagnostics, and genetic testing. Students undertake the large-scale production, isolation, and purification of DNA and proteins from microorganisms and eukaryotic cells by using a fermentor, high-speed centrifuge, and homogenizer, and they characterize these molecules by polyacrylamide and agarose gel electrophoresis. The students also explore technologies of molecular probing by fluorescence microscopy and Southern and Western blotting with nonradioactive probes.

9452336霍普金斯实验室在分子和细胞生物学方面的经验通过引入新的实验和向更多的学生提供现有的实验而得到加强。这些经验对生物和化学专业的学生以及科学教育和护理专业的学生都有好处。为学生创造在研究、诊断和基因测试中使用的技术方面的实践经验的机会。学生使用发酵罐、高速离心机和匀浆机从微生物和真核细胞中大规模生产、分离和纯化DNA和蛋白质，并用聚丙烯酰胺和琼脂糖凝胶电泳法对这些分子进行表征。学生们还探索了用荧光显微镜进行分子探测以及用非放射性探针进行Southern和Western印迹的技术。