Analysis and Selection of Microencapsulated Transfected Cells

这个小型企业创新研究第一阶段项目旨在评估One Cell Systems的微胶囊技术在转基因细胞评估中的使用。目前，转基因或转基因细胞在开发疫苗、诊断或药物输送系统以及治疗恶性、传染性和遗传性疾病方面的商业重要性已得到充分认识。获得并鉴定一个纯的转基因细胞群体或评估不同的转基因方法的效果通常需要包括耐药基因和克隆选择。将单个细胞包裹到琼脂糖微滴中的能力将允许使用自动方法，如流式细胞术和荧光激活细胞分选，在单个细胞中测量所需蛋白质的表达。这项建议旨在确定使用包埋系统评估转基因细胞的可行性。这些初步研究将利用两个不同的转基因细胞系：中国仓鼠卵巢细胞系(CHO L-15500)。分泌组织型纤溶酶原激活剂的ATCC CRL 9609)和分泌人绒毛膜促性腺激素的小鼠乳腺株(CSM a b Lh，ATCC CRL 8401)。这些第一阶段研究的成功完成将为开发一种新的、可能更有效的评估和选择转基因细胞的技术提供基础。

This Small Business Innovation Research Phase I project is designed to evaluate the use of One Cell Systems' microencapsulation technology in the assessment of transfected cells. The current commercial importance of genetically modified or transfected cells in the development of vaccines, diagnostics or drug delivery systems as well as the treatment of malignant, infectious and genetic diseases is well recognized. Obtaining and characterizing a pure population of transfected cells or evaluating the efficacy of different transfection methods usually requires including a drug-resistance gene and clone selection. The ability to encapsulate single cells into agarose microdrops would allow the expression of the desired protein to be measured in individual cells using automated methods such as flow cytometry and fluorescence-activated cell sorting. This proposal is designed to determine the feasibility of using the encapsulation system to evaluate transfected cells. These initial studies will utilize two different transfected cell lines; a Chinese hamster ovary cell line (CHO l-l5500. ATCC CRL 9609) which secretes tissue plasminogen activator and a mouse mammary line (CSM a b lH, ATCC CRL 8401) secreting human chorionic gonadotropin. Successful completion of these phase I studies would provide the basis for the development of a new and potentially more efficient technology for the evaluation and selection of transfected cells.