SBIR Phase II: Indetification of Anaerobic Bacteria and their Metalbolic Capabilities for Bioremediation

SBIR 第二阶段：厌氧细菌及其生物修复代谢能力的鉴定

• 批准号: 9500041
• 负责人: Barry Bochner
• 金额: $ 30万
• 依托单位: BIOLOG INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-10-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9500041&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Indetification; Anaerobic

9500041 Bochner Anaerobic bacteria possess metabolic activities for biodegrading a range of polluting chemicals including aromatic, halogenated aromatic, and halogenated alkene compounds. Anaerobes make up a significant portion of terrestrial bacteria and should be considered as potential agents of bioremediation. However, knowledge of these organisms, their diversity, taxonomy, and metabolic capabilities, is still at a rudimentary level. The purpose of this project is to design an efficient, easy-to-use, instrumented test kit system that can be used both to study the catabolic capabilities of anaerobes and to identify different species of anaerobes based on these capabilities. To develop this kit, the catabolic potential of over 300 species of anaerobes will be surveyed and cataloged against at least 300 different organic compounds, including some important xenobiotic chemicals. From computer analysis of these data, a set of 95 carbon source utilization tests will be selected to be incorporated into a commercial microplate test kit. The survey will provide an overview of the range of catabolic activities of the anaerobes in question. If successful, the test kit system will be marketed commercially to microbiologists doing environmental, industrial, and medical testing and identification of anaerobes.

Bochner厌氧菌具有生物降解一系列污染化学物质的代谢活性，包括芳香族、卤代芳香族和卤代烯烃化合物。厌氧菌是陆生细菌的重要组成部分，是一种潜在的生物修复剂。然而，关于这些生物的知识，它们的多样性，分类和代谢能力，仍然处于初级水平。该项目的目的是设计一个高效，易于使用的仪器测试试剂盒系统，既可以用来研究厌氧菌的分解代谢能力，又可以根据这些能力来识别不同种类的厌氧菌。为了开发该试剂盒，将对300多种厌氧菌的分解代谢潜力进行调查，并对至少300种不同的有机化合物进行分类，包括一些重要的异种化学物质。从对这些数据的计算机分析中，将选出一套95个碳源利用试验，纳入商业微孔板试验试剂盒。该调查将提供厌氧菌分解代谢活动范围的概述。如果成功，测试套件系统将商业化销售给微生物学家做环境，工业和医学测试和鉴定厌氧菌。