Identification of Anaerobic Bacteria and Their Metabolic Capabilities for Bioremediation

9362058 Bochner厌氧菌具有生物降解一系列污染化学品的代谢活性，包括芳香化学品，卤代芳烃和卤代烯烃。厌氧菌是陆生细菌的重要组成部分，应该被认为是生物修复的潜在媒介，但我们对这些生物的认识，它们的多样性，分类和代谢能力，仍然处于初级水平。将开发一种高效、易于使用的仪器检测试剂盒系统，该系统既可用于研究厌氧菌的分解代谢能力，又可根据这些能力识别不同种类的厌氧菌。300多种厌氧菌对近200种有机化学品的分解代谢潜力将被调查和编目。从对这些数据的计算机分析中，将选择一套95个碳源利用测试，最终将其纳入商业微孔板测试试剂盒。该调查将提供厌氧菌分解代谢活动范围的概述，仪器测试试剂盒系统将商业化销售给从事环境、工业和医学厌氧菌测试和鉴定的微生物学家。＊＊＊

9362058 Bochner Anaerobic bacteria possess metabolic activities for biodegrading a range of polluting chemicals including aromatic chemicals, halogenated aromatics, and halogenated alkenes. Anaerobes make up a significant portion of terrestrial bacteria and should be considered as potential agents of bioremediation, but our knowledge of these organisms, their diversity, taxonomy, and metabolic capabilities, is still at a rudimentary level. An efficient, easy- to-use, instrumented test kit system that can be used both to study the catabolic capabilities of anaerobes and to identify different species of anaerobes based on those capabilities will be developed. The catabolic potential of over 300 species of anaerobes against nearly 200 organic chemicals will be surveyed and cataloged. From computer analysis of this data, a set of 95 carbon source utilization tests will be selected that ultimately will be incorporated into a commercial microplate test kit. %%% The survey will provide an overview of the range of catabolic activities of anaerobes, and the instrumented test kit system will be marketed commercially to microbiologists doing environmental, industrial, and medical testing and identification of anaerobes. ***