Receptor-Mediated Cellular Adhesion: Measurement of Bond Strength and Elasticity

受体介导的细胞粘附：粘合强度和弹性的测量

• 批准号: 9501145
• 负责人: Dennis Prieve
• 金额: $ 17.49万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-15 至 1998-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9501145&HistoricalAwards=false
• 关键词: Receptor; Mediated; Cellular; Adhesion; Measurement

9501145 Prieve This project is on research to use total internal reflection microscopy (TIRM), which was developed in the Principal Investigator's (PI) laboratory to measure colloidal interactions, to determine: (1) the forces between single receptor-ligand pairs; and (2) the elasticity of single bonds. In addition, the magnitudes of forces at large separations will be correlated with the measured adhesion between the receptor and ligand coated surfaces. The model cells for the adhesion experiments will be immunoglobulin-G-coated latex beads and a protein-A-coated glass plate. ***

9501145 Prieve该项目是关于使用全内反射显微镜（TIRM）的研究，TIRM是在主要研究者（PI）实验室开发的，用于测量胶体相互作用，以确定：（1）单个受体-配体对之间的力;和（2）单键的弹性。 此外，在大的分离力的大小将与受体和配体涂覆的表面之间的测量的粘附力相关。 用于粘附实验的模型细胞将是免疫球蛋白G包被的乳胶珠和蛋白A包被的玻璃板。 ***