Receptor-Mediated Cellular Adhesion: Measurement of Bond Strength and Elasticity

9501145前夕，本项目正在研究使用首席研究员实验室开发的全内反射显微镜(TIRM)来测量胶体相互作用，以确定：(1)单个受体-配体对之间的作用力；以及(2)单键的弹性。此外，大间隔时的力的大小将与受体和配体涂层表面之间测量的粘附性相关。黏附实验的模型细胞将是免疫球蛋白-G涂层的乳胶珠和A蛋白涂层的玻璃板。***

9501145 Prieve This project is on research to use total internal reflection microscopy (TIRM), which was developed in the Principal Investigator's (PI) laboratory to measure colloidal interactions, to determine: (1) the forces between single receptor-ligand pairs; and (2) the elasticity of single bonds. In addition, the magnitudes of forces at large separations will be correlated with the measured adhesion between the receptor and ligand coated surfaces. The model cells for the adhesion experiments will be immunoglobulin-G-coated latex beads and a protein-A-coated glass plate. ***