SIMULATION OF RECEPTOR-LIGAND-MEDIATED CELLULAR ADHESION IN A LINEAR SHEAR FIEL

线性剪切场中受体-配体介导的细胞粘附的模拟

• 批准号: 7956360
• 负责人: CHARLES Dionisio EGGLETON
• 金额: $ 0.08万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956360
• 关键词: Adhesives; Biomedical Research; Caliber; Cardiovascular system; Cell Adhesion; Cells; Computer Retrieval of Information on Scientific Projects Database; Coupling; Funding; Grant; High Performance Computing; Institution; Length; Leukocytes; Ligands; Light; Liquid substance; Mediating; Membrane; Methods; Modeling; Physiological Processes; Play; Process; Research; Research Personnel; Resources; Role; Simulate; Source; United States National Institutes of Health; hemodynamics; neutrophil; receptor; simulation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell adhesion in the presence of hemodynamic flow plays a fundamental role in many of the physiological processes occurring in the circulatory system. These adhesive interactions are influenced by processes taking place at drastically different length scales: (1) Fluid forces which tend to deform the cells are on the length scale of the cell diameter (~10 ?m) whereas (2) the range of receptor-ligand bonds mediating cell adhesion is ~ 100 nm. The coupling of forces acting at these disparate length scales as well as their effects on cell deformation makes the simulation of cell adhesion computationally intensive. We simulate the homotypic aggregation of polymorphonuclear leukocytes in a linear shear field using the immersed boundary method with a stochastic description of receptor-ligand interactions. The cells have been modeled as spherical Neo-Hookean membranes enclosing a Newtonian fluid. This study will shed light on the effects of fluid shear and cell deformability on the efficiency of leukocyte aggregation.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 血流动力学流动下的细胞粘附在循环系统中发生的许多生理过程中起着基本作用。这些粘附相互作用受到在截然不同的长度尺度上发生的过程的影响：（1）倾向于使细胞变形的流体力在细胞直径的长度尺度上（〜10微米），而（2）介导细胞粘附的受体-配体键的范围是〜100纳米。在这些不同长度尺度上作用的力的耦合及其对细胞变形的影响使得细胞粘附的模拟计算量很大。我们使用浸入边界法和受体-配体相互作用的随机描述来模拟线性剪切场中多形核白细胞的同型聚集。这些细胞被建模为包围牛顿流体的球形新胡克膜。这项研究将揭示流体剪切和细胞变形性对白细胞聚集效率的影响。