SIMULATION OF RECEPTOR-LIGAND-MEDIATED CELLULAR ADHESION IN A LINEAR SHEAR FIEL

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 在血液动力学流动存在下的细胞粘附在循环系统中发生的许多生理过程中起着重要作用。这些粘附相互作用的影响过程发生在完全不同的长度尺度：（1）流体的力量，往往变形的细胞是在细胞直径的长度尺度（~10？m）而（2）介导细胞粘附的受体-配体键的范围约为100 nm。在这些不同的长度尺度上作用的力的耦合以及它们对细胞变形的影响使得细胞粘附的模拟计算密集。我们模拟同型聚集的多形核白细胞在线性剪切场使用浸没边界方法与随机描述的受体-配体相互作用。细胞已被建模为封闭的牛顿流体的球形Neo-Hookean膜。本研究将阐明流体剪切力和细胞变形性对白细胞聚集效率的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell adhesion in the presence of hemodynamic flow plays a fundamental role in many of the physiological processes occurring in the circulatory system. These adhesive interactions are influenced by processes taking place at drastically different length scales: (1) Fluid forces which tend to deform the cells are on the length scale of the cell diameter (~10 ?m) whereas (2) the range of receptor-ligand bonds mediating cell adhesion is ~ 100 nm. The coupling of forces acting at these disparate length scales as well as their effects on cell deformation makes the simulation of cell adhesion computationally intensive. We simulate the homotypic aggregation of polymorphonuclear leukocytes in a linear shear field using the immersed boundary method with a stochastic description of receptor-ligand interactions. The cells have been modeled as spherical Neo-Hookean membranes enclosing a Newtonian fluid. This study will shed light on the effects of fluid shear and cell deformability on the efficiency of leukocyte aggregation.