Cellular and Molecular Interactions Between Symbiotic Chloroplasts and Molluscan Cells

小行星9505416 该提案描述了几个旨在了解海洋蛞蝓Elysia chlorotica细胞用于捕获海洋蛞蝓Vaucheria litorea叶绿体的机制的实验，并在捕获后长达8个月的时间内保持质体在动物细胞内的光合作用活性。 光合作用需要某些叶绿体蛋白质不断地被替换。 合成这些蛋白质的遗传信息包含在叶绿体中，但其他蛋白质通常由植物细胞核提供。 这些实验将使用放射性标记和凝胶电泳技术，以表明当质体在动物细胞内时，哪些叶绿体蛋白质是合成的。 使用药理学试剂，将确定合成蛋白质的遗传信息的位点（叶绿体或动物细胞核）。 抗体标记将用于鉴定合成的蛋白质。 这些结果将开始提供信息，从而了解动物细胞如何在功能条件下保持完全外源的结构（叶绿体）。 ***

9505416 Pierce This proposal describes several experiments designed to understand the mechanism(s) that are used by the cells of the marine slug, Elysia chlorotica, to capture chloroplasts from the marine alga, Vaucheria litorea, and keep the plastids photosynthetically active within the animal cell for periods as long as 8 months after capture. The photosynthesis requires that certain chloroplast proteins be continually replaced. The genetic information for the synthesis of some of those proteins is contained within the chloroplast, but for other of the proteins is normally supplied by the plant nucleus. The experiments, which will use radiolabelling and gel electrophoresis techniques, will indicate which of the chloroplast proteins are synthesized while the plastid is within the animal cell. Using pharmacological agents, the site (chloroplast or animal nucleus) of the genetic information for the synthesized proteins will be determined. Antibody labelling will be used to identify the synthesized proteins. The results will begin to provide information leading to understanding how the animal cell maintains within it a completely foreign structure (chloroplast) in a functional condition. ***