Cellular and Molecular Interactions Between Symbiotic Chloroplasts and Molluscan Cells

9505416皮尔斯这项建议描述了几个旨在了解海洋鼻涕虫细胞(S)用来从海藻Vucheria litorea捕获叶绿体并在捕获后长达8个月的时间内保持动物细胞内的光合作用活性的机制的实验(S)。光合作用需要不断地替换某些叶绿体蛋白质。合成其中一些蛋白质的遗传信息包含在叶绿体中，但对于其他蛋白质，通常是由植物细胞核提供的。这些实验将使用放射性标记和凝胶电泳技术，将表明当叶绿体在动物细胞内时，哪些叶绿体蛋白是合成的。利用药理学试剂，将确定合成蛋白质的遗传信息的位置(叶绿体或动物核)。抗体标记将用于鉴定合成的蛋白质。这些结果将开始提供信息，帮助理解动物细胞如何在功能状态下维持一个完全外来的结构(叶绿体)。***

9505416 Pierce This proposal describes several experiments designed to understand the mechanism(s) that are used by the cells of the marine slug, Elysia chlorotica, to capture chloroplasts from the marine alga, Vaucheria litorea, and keep the plastids photosynthetically active within the animal cell for periods as long as 8 months after capture. The photosynthesis requires that certain chloroplast proteins be continually replaced. The genetic information for the synthesis of some of those proteins is contained within the chloroplast, but for other of the proteins is normally supplied by the plant nucleus. The experiments, which will use radiolabelling and gel electrophoresis techniques, will indicate which of the chloroplast proteins are synthesized while the plastid is within the animal cell. Using pharmacological agents, the site (chloroplast or animal nucleus) of the genetic information for the synthesized proteins will be determined. Antibody labelling will be used to identify the synthesized proteins. The results will begin to provide information leading to understanding how the animal cell maintains within it a completely foreign structure (chloroplast) in a functional condition. ***