Request for a Scanning Spectrofluorimeter

索取扫描分光荧光计

• 批准号: 9002078
• 负责人: Sidney Pierce
• 金额: $ 5.9万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-06-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9002078&HistoricalAwards=false
• 关键词: Request; Scanning; Spectrofluorimeter

This proposal requests funds to obtain a scanning spectrofluorimeter, associated epifluorescense microscope and computer. The instrumentation will be used in the research programs of several investigators, all of whom need to measure intracellular ion concentrations using various ion sensitive dyes. The specific projects in this proposal will investigate the role of intracellular calcium in cell volume recovery by invertebrate blood cells following a hypoosmotic stress; the role of calcium in acetylcholine receptor differentiation; the role of calcium in visual transduction in Limulus eye and vertebrate retina; the role of calcium fluctuations in the regulation of mitosis in plants.

该提案要求提供资金， 荧光分光光度计、相关落射荧光显微镜和 电脑 该仪器将用于研究 几个研究人员的项目，他们都需要测量 细胞内离子浓度使用各种离子敏感 染料。 本提案中的具体项目将进行调查 细胞内钙离子在细胞体积恢复中作用 低渗应激后无脊椎动物血细胞;作用 乙酰胆碱受体分化中钙的作用 钙在鲎眼和脊椎动物视觉信号转导中作用 视网膜;钙波动在调节 植物的有丝分裂