Design of Reaction Catalysts Based on Fatty Acid Binding Protein

Mark D博士Distefano，明尼苏达大学化学系， 双子城，是由无机，生物无机， 化学系有机化学专业 教师早期职业发展奖设计半合成酶 其将催化有机分子的区域选择性转化， 在研究生阶段进行课程和课程开发， 本科水平，并从事社区外展计划。通过 将过渡金属催化剂连接至脂肪酸结合蛋白，以及 通过在其内部引入噻唑杂环， 蛋白质的类型，区域和立体选择性氧化的催化剂 聚烯烃种类和对映选择性碳-碳 债券 将准备形成反应。利用蛋白质获得催化剂 在没有共价修饰的情况下，辅因子结合结构域将被 通过诱变或通过融合酶引入蛋白质中。使用 当前教育理论的一个大的有机化学课程和 研究生课程的反应机制将重新设计，以增加 这些经验的有效性和智力刺激。到 向当地学校传达科学推广计划的兴奋之处 将扩大。 许多合成化学品的生产过程涉及有毒物质 材料和由此产生的废物的处理已经产生了 巨大的环境问题。解决这些困难的一个办法是 使用酶（基于蛋白质的催化剂）来催化化学反应。 该项目的目标是制备新的酶， 重要的化学变化。这个项目的成功将导致 制备能够 进行非常有效的转化， 你知道的。教育活动旨在增加 学生欣赏化学，并将涉及学童， 研究生。

Dr. Mark D. Distefano, Chemistry Department, University of Minnesota - Twin Cities, is supported by the Inorganic, Bioinorganic, and Organometallic Chemistry Program of the Chemistry Division under a Faculty Early Career Advancement Award to design semi-synthetic enzymes which will catalyze regioselective transformations of organic molecules, to undertake course and curriculum development at the graduate and undergraduate level, and to engage in community outreach programs. By attaching a transition metal catalyst to a fatty acid binding protein and by introducing a thiazolium heterocycle into the interior of the same type of protein, catalysts for the regio- and stereo-selective oxidation of polyolefinic species and for enantioselective carbon-carbon bond forming reactions will be prepared. To obtain catalysts using proteins without covalent modification, cofactor binding domains will be introduced into the protein by mutagenesis or by fusion enzymes. Using current educational theory a large organic chemistry course and a graduate course in reaction mechanisms will be redesigned to increase the effectiveness and intellectual stimulation of these experiences. To communicate the excitement of science out-reach programs to local schools will be expanded. Many synthetic chemicals are made using processes that involve toxic materials and the disposal of the resulting waste products has created enormous environmental problems. One solution to these difficulties employs enzymes (protein based catalysts) to catalyze chemical reactions. The goal of this project is to prepare new enzymes which will accomplish important chemical transformations. Success in this project would lead to the ability to prepare semi-synthetic enzymes which are capable of performing very efficient transformations for which no natural enzymes are know. The educational activities are designed toward increasing student appreciation of chemistry and will involve school children to graduate students.