Design of Reaction Catalysts Based on Fatty Acid Binding Protein

基于脂肪酸结合蛋白的反应催化剂设计

• 批准号: 9506793
• 负责人: Mark Distefano
• 金额: $ 22.23万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-06-01 至 1998-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9506793&HistoricalAwards=false
• 关键词: Design; Reaction; Catalysts; Based; Fatty

Dr. Mark D. Distefano, Chemistry Department, University of Minnesota - Twin Cities, is supported by the Inorganic, Bioinorganic, and Organometallic Chemistry Program of the Chemistry Division under a Faculty Early Career Advancement Award to design semi-synthetic enzymes which will catalyze regioselective transformations of organic molecules, to undertake course and curriculum development at the graduate and undergraduate level, and to engage in community outreach programs. By attaching a transition metal catalyst to a fatty acid binding protein and by introducing a thiazolium heterocycle into the interior of the same type of protein, catalysts for the regio- and stereo-selective oxidation of polyolefinic species and for enantioselective carbon-carbon bond forming reactions will be prepared. To obtain catalysts using proteins without covalent modification, cofactor binding domains will be introduced into the protein by mutagenesis or by fusion enzymes. Using current educational theory a large organic chemistry course and a graduate course in reaction mechanisms will be redesigned to increase the effectiveness and intellectual stimulation of these experiences. To communicate the excitement of science out-reach programs to local schools will be expanded. Many synthetic chemicals are made using processes that involve toxic materials and the disposal of the resulting waste products has created enormous environmental problems. One solution to these difficulties employs enzymes (protein based catalysts) to catalyze chemical reactions. The goal of this project is to prepare new enzymes which will accomplish important chemical transformations. Success in this project would lead to the ability to prepare semi-synthetic enzymes which are capable of performing very efficient transformations for which no natural enzymes are know. The educational activities are designed toward increasing student appreciation of chemistry and will involve school children to graduate students.

明尼苏达大学双城分校化学系的 Mark D. Distefano 博士得到了化学系无机、生物无机和有机金属化学项目的支持，该项目获得了教师早期职业发展奖，旨在设计半合成酶，以催化有机分子的区域选择性转化，并在研究生和本科生阶段进行课程和课程开发， 并参与社区外展计划。通过将过渡金属催化剂附着到脂肪酸结合蛋白上，并通过将噻唑鎓杂环引入到同类型蛋白质的内部，将制备用于聚烯烃物种的区域选择性和立体选择性氧化以及用于对映选择性碳-碳键形成反应的催化剂。为了使用未经共价修饰的蛋白质获得催化剂，将通过诱变或融合酶将辅因子结合域引入蛋白质中。利用当前的教育理论，将重新设计大型有机化学课程和反应机制研究生课程，以提高这些体验的有效性和智力刺激。将扩大向当地学校传达科学推广计划的兴奋点。 许多合成化学品的制造过程都涉及有毒物质，而产生的废物的处理造成了巨大的环境问题。解决这些困难的一种方法是使用酶（基于蛋白质的催化剂）来催化化学反应。 该项目的目标是制备能够完成重要化学转化的新酶。该项目的成功将导致能够制备半合成酶，这些酶能够进行非常有效的转化，而天然酶尚不清楚。这些教育活动旨在提高学生对化学的欣赏，并将让小学生和研究生参与其中。