Functional Analysis of the Late Endosome and Associated Rab Proteins

MCB 9507206 Angela Wandinger-Ness 本项目研究内吞途径中的膜转运机制以及晚期内体在转运和催化特定反应中的功能。 为此，生物化学和遗传学方法的组合将被用来表征特定形式的rab 7与肌动蛋白细胞骨架的相互作用。 酵母双杂交系统将被用来确定相互作用的合作伙伴的rab 7作为一个必然的方法。 此外，将开发一种体外测定系统，以研究晚期内吞膜转运。 后一种分析将通过开发透化系统来扩展，该系统检查rab 7及其合作伙伴之间的相互作用机制。 这个项目是一个教师早期职业发展奖，也需要一个教育计划。 在本项目的教育部分，开发了一种在本科课堂上进行主动学习的方法。 此外，还将向学生介绍职业培训计划，使他们更好地适应就业市场。 最后，该项目还包括对大学预科教育工作者的宣传。 %本项目将研究组件被定向到单元内特定位置的机制。 它将有助于解释食物等分子如何进入细胞并最终到达消化的正确位置。 该项目的教育计划将有助于加强获奖机构的本科教育，并使该机构的本科生更好地适应就业市场。 ***

MCB 9507206 Angela Wandinger-Ness This project examines the mechanisms of membrane transport in the endocytic pathway and the function of the late endosome in both transport and in catalyzing specialized reactions. To this end, a combination of biochemical and genetic approaches will be used to characterize the interaction of specific forms of rab7 with the actin cytoskeleton. The yeast two hybrid system will be employed to identify interacting partners of rab7 as a corollary approach. In addition, an in vitro assay system will be developed to study late endocytic membrane transport. This latter analysis will be extended by the development of a permeabilized system which examines the mechanisms of interaction between rab7 and its partners. This project is a Faculty Early Career Development Award and also entail an education plan. In the Education portion of this project an approach is developed for active learning in the undergraduate classroom. In addition, introduction to career-training programs will be provided to the students to make them better suited to the job market. Finally, the project includes outreach to pre-college educators. %%% This project will examine the mechanisms by which components are directed to specific locations within the cell. It will help explain how molecules such as food enter the cell and end up in the correct location for digestion. The education plan of this project will serve to strengthen the undergraduate education at the awardee's institution, and to make the undergraduates at the institution better suited to the job market. ***