Modifiers of Dynein Function in Drosophila

本研究旨在探讨动力蛋白运动复合物在胚胎细胞和体细胞中的功能。研究者将分离和表征显性“粘合”基因的外基因增强子和抑制子，并将从果蝇胚胎中纯化mrnp -动力蛋白-微管复合物。将进行基因敲除实验来测试细胞质动力蛋白的作用。这项工作将与明尼苏达大学的Thomas Hays博士合作进行。分子马达用于移动细胞内的部件，就像船上的舷外马达使船在水中行驶一样。申请人将采用可能的果蝇遗传方法来了解马达是如何工作的。* * *

Abstract K. Supernant The proposed research is an investigation of how the dynein motor complex functions in embryonic and somatic cells. The investigator will isolated and characterize extragenic enhancers and suppressors of the dominant "glued" gene and will purify mRNP-dynein-microtubule complexes from Drosophila embryos. Genetic knockout experiments will be conducted to test the role of cytoplasmic dynein. This work will be performed in association with Dr. Thomas Hays at the University of Minnesota. %%% Molecular motors serve to move components within cells much like a outboard motor on a boat moves the boat through the water. The applicant will employ genetic approaches possible in the fruit fly Drosophila to understand how the motor works. ***