Self-Assembled Monolayers from Alkanethiolate-tethered Osmium and Ruthenium Complexes. Properties and Applications in Sensors

这项研究计划资助项目涉及材料化学的广泛领域，对生物技术具有重大影响。在这项为期18个月的标准拨款期间，雷诺内华达大学的-张教授和她的学生将进行必要的初步实验，以实施一项基于自组装单层驻留受体的分子识别和互补发光检测的生物传感器技术的重大研究计划。制备了硫代烷基络合物，并将其引入到金表面的硫醇自组装单分子膜中，表征了它们的化学发光和电致化学发光性质。还将制备掺入感兴趣的DNA序列的拴系甲基膦酸寡核苷酸类似物的混合SAM，并将确定双链DNA与拴系类似物相互作用的程度，并改变来自Ru和Om配合物的发光。该研究计划资助项目的目标是探索含Ru和Os络合物的自组装膜的发光特性，并利用该实验平台作为新的序列特定DNA传感器的基础。这一跨学科项目可能带来具有重要生物技术、环境和临床应用的传感器技术。

This Research Planning Grant project is in the broad area of materials chemistry and has significant implications for biotechnology. During the tenure of this eighteen-month standard grant, Professor Tam-Chang and her students at the University of Nevada, Reno will carry out preliminary experiments necessary to implement a major research program in biosensor technology based on molecular recognition by self-assembled monolayer-resident receptors and complementary luminescence detection. Alkanethiolate-tethered ruthenium and osmium complexes will be prepared and incorporated into self-assembled monolayers (SAMs) of alkanethiols on gold surfaces, and their chemiluminescence and electrochemiluminescence properties characterized. Mixed SAMs in which tethered methylphosphonate oligonucleotide analogs of DNA sequences of interest are also incorporated will be prepared and the extent to which double-stranded DNA interacts with the tethered analog and alters the luminescence from the ruthenium and osmium complexes will be determined. The objective of this Research Planning Grant project is to explore the luminescence properties of SAMs containing ruthenium and osmium complexes, and to utilize that experimental platform as the basis for new sequence-specific DNA sensors. This interdisciplinary project could give rise to sensor technology with important biotechnological, environmental, and clinical applications.