Self-Assembled Monolayers from Alkanethiolate-tethered Osmium and Ruthenium Complexes. Properties and Applications in Sensors

本研究计划资助项目涉及材料化学的广泛领域，并对生物技术有重大影响。在这项为期18个月的标准资助期内，张丹教授和她在内华达大学里诺分校的学生将进行必要的初步实验，以实施一项基于自组装单层驻留受体和互补发光检测的分子识别生物传感器技术的重大研究项目。制备链烷硫醇系链钌和锇配合物，并将其结合到金表面的链烷硫醇自组装单层（SAMs）中，并对其化学发光和电化学发光性能进行了表征。将制备混合SAMs，其中还包含感兴趣的DNA序列的系链甲基膦酸寡核苷酸类似物，并且将确定双链DNA与系链类似物相互作用并改变钌和锇配合物发光的程度。本研究计划资助项目的目的是探索含有钌和锇配合物的钐的发光特性，并利用该实验平台作为新的序列特异性DNA传感器的基础。这个跨学科的项目可以产生具有重要生物技术，环境和临床应用的传感器技术。

This Research Planning Grant project is in the broad area of materials chemistry and has significant implications for biotechnology. During the tenure of this eighteen-month standard grant, Professor Tam-Chang and her students at the University of Nevada, Reno will carry out preliminary experiments necessary to implement a major research program in biosensor technology based on molecular recognition by self-assembled monolayer-resident receptors and complementary luminescence detection. Alkanethiolate-tethered ruthenium and osmium complexes will be prepared and incorporated into self-assembled monolayers (SAMs) of alkanethiols on gold surfaces, and their chemiluminescence and electrochemiluminescence properties characterized. Mixed SAMs in which tethered methylphosphonate oligonucleotide analogs of DNA sequences of interest are also incorporated will be prepared and the extent to which double-stranded DNA interacts with the tethered analog and alters the luminescence from the ruthenium and osmium complexes will be determined. The objective of this Research Planning Grant project is to explore the luminescence properties of SAMs containing ruthenium and osmium complexes, and to utilize that experimental platform as the basis for new sequence-specific DNA sensors. This interdisciplinary project could give rise to sensor technology with important biotechnological, environmental, and clinical applications.