Self-Assembled Monolayers from Alkanethiolate-tethered Osmium and Ruthenium Complexes. Properties and Applications in Sensors

这个研究规划补助金项目是在材料化学的广泛领域，对生物技术有重大影响。 在十八个月的标准资助期内，内华达州大学里诺分校的谭-张教授及其学生将进行初步实验，以实施一项基于自组装单分子层受体分子识别和互补发光检测的生物传感器技术的重大研究计划。 烷硫醇栓系钌和锇配合物的制备和纳入自组装单分子膜（SAMs）的烷硫醇在金表面，其化学发光和电化学发光特性的特点。 将制备其中还掺入了感兴趣的DNA序列的拴系甲基膦酸酯寡核苷酸类似物的混合SAM，并将确定双链DNA与拴系类似物相互作用并改变钌和锇络合物发光的程度。 该研究计划资助项目的目的是探索含有钌和锇络合物的自组装膜的发光特性，并利用该实验平台作为新的序列特异性DNA传感器的基础。 这个跨学科的项目可能会产生具有重要生物技术，环境和临床应用的传感器技术。

This Research Planning Grant project is in the broad area of materials chemistry and has significant implications for biotechnology. During the tenure of this eighteen-month standard grant, Professor Tam-Chang and her students at the University of Nevada, Reno will carry out preliminary experiments necessary to implement a major research program in biosensor technology based on molecular recognition by self-assembled monolayer-resident receptors and complementary luminescence detection. Alkanethiolate-tethered ruthenium and osmium complexes will be prepared and incorporated into self-assembled monolayers (SAMs) of alkanethiols on gold surfaces, and their chemiluminescence and electrochemiluminescence properties characterized. Mixed SAMs in which tethered methylphosphonate oligonucleotide analogs of DNA sequences of interest are also incorporated will be prepared and the extent to which double-stranded DNA interacts with the tethered analog and alters the luminescence from the ruthenium and osmium complexes will be determined. The objective of this Research Planning Grant project is to explore the luminescence properties of SAMs containing ruthenium and osmium complexes, and to utilize that experimental platform as the basis for new sequence-specific DNA sensors. This interdisciplinary project could give rise to sensor technology with important biotechnological, environmental, and clinical applications.