权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

RUI: Development Analysis of Forebrain

RUI：前脑发育分析

基本信息

批准号：
9511042
负责人：
Gerald Eagleson
金额：
$ 19.83万
依托单位：
Loras College
依托单位国家：
美国
项目类别：
Continuing grant
财政年份：
1995
资助国家：
美国
起止时间：
1995-09-01 至 1999-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9511042&HistoricalAwards=false
关键词：
RUI Development Analysis Forebrain

项目摘要

9511042 Eagleson This research will investigate early forebrain development in the vertebrate. The animal to be used was chosen because a mutation found within this species directly affects forebrain and eye development, and this mutation provides a negative condition whereby development is disrupted. Using normal and mutant organisms, a detailed forebrain fate map will be produced with fluorescent labeling techniques. A comparison of the mutant and normal embryo neural plate brain fates will test whether, and when, specific midline early neural plate tissue is defective in the mutant embryos. Using in situ hybridization techniques, stage by stage gene expression changes in forebrain specific genes will be followed in normal and mutant embryos. These studies will test to see if differences in the timing or amount of forebrain specific gene expression occurs in mutant and normal embryos, and if gene expression events can be related to the specific forebrain defects. Additional studies will test how the mutant condition might alter later axon growth and neural connections. These studies will follow axon and synapse development in normal and mutant embryos by using immunocytochemistry for a protein that is formed in developing neurons. These studies will reveal how a single mutant gene can result in defects that manifest themselves much later in development. In addition these studies will provide a developmental framework and model for further studies investigating vertebrate neural development.

9511042 Angleson本研究将研究脊椎动物的早期前脑发育。选择使用的动物是因为在该物种中发现的突变直接影响前脑和眼睛发育，并且该突变提供了发育被破坏的负面条件。使用正常和突变的生物体，一个详细的前脑命运地图将与荧光标记技术。突变体和正常胚胎神经板脑命运的比较将测试特定中线早期神经板组织在突变体胚胎中是否以及何时有缺陷。使用原位杂交技术，在正常和突变胚胎中，前脑特异性基因的阶段性基因表达变化将被跟踪。这些研究将测试突变和正常胚胎中前脑特异性基因表达的时间或数量是否存在差异，以及基因表达事件是否与特定的前脑缺陷有关。进一步的研究将测试突变条件如何改变以后的轴突生长和神经连接。这些研究将通过对发育中的神经元中形成的一种蛋白质进行免疫细胞化学，来跟踪正常和突变胚胎中轴突和突触的发育。这些研究将揭示单个突变基因如何导致缺陷，这些缺陷在发育后期表现出来。此外，这些研究将为进一步研究脊椎动物神经发育提供一个发展框架和模型。