Progesterone and the Neural Mechanisms of Behavior

黄体酮和行为的神经机制

• 批准号: 9511895
• 负责人: Joseph DeBold
• 金额: $ 24.08万
• 依托单位: Tufts University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9511895&HistoricalAwards=false
• 关键词: Progesterone; Neural; Mechanisms; Behavior

Proposal Number: IBN-9511895 Principal Investigator: DeBold, Joseph F. Progesterone is an important steroid hormone for regulating many aspects of physiology and behavior. According to the classical approach, steroid hormones exert their effects by entering cells, binding with a receptor inside the cell, and then the steroid-receptor complex acts on specific sites on the chromosomes to change which proteins that cell is producing. This mechanism of action has been described in virtually every cell that responds to steroid hormones. However, it appears to be absent in the ventral midbrain. Dr. DeBold's previous studies have shown that a form of progesterone that cannot enter neurons is effective in altering behavior when administered in the midbrain but not in other steroid sensitive areas. Therefore, the primary focus of the proposed studies is to determine how progesterone is acting on neurons to influence behavior and the connectivity of these neurons. Dr. DeBold will identify the neurons containing these unique characteristics by labeling progesterone with a fluorescent tag and identifying the cell to which it binds and then using neurochemical procedures, characterize the type of cells containing these novel receptors. Using selective pharmacological agents, the role of other neurotransmitters in mediating progesterone actions will be established. These studies investigate novel effects of steroid hormones and will be extremely beneficial in understanding the physiology and pathophysiology of these essential molecules.

主要研究者：DeBold， Joseph F.黄体酮是一种重要的类固醇激素，用于调节生理和行为的许多方面。根据经典方法，类固醇激素通过进入细胞，与细胞内的受体结合发挥作用，然后类固醇受体复合物作用于染色体上的特定位置，改变细胞产生的蛋白质。这种作用机制在几乎所有对类固醇激素有反应的细胞中都有描述。然而，它似乎在腹侧中脑中缺失。德博尔德博士之前的研究表明，一种不能进入神经元的黄体酮在中脑使用时可以有效地改变行为，但在其他类固醇敏感区域则没有效果。因此，拟建研究的主要焦点是确定黄体酮如何作用于神经元以影响这些神经元的行为和连接。德博尔德博士将通过用荧光标记黄体酮来识别含有这些独特特征的神经元，并识别其结合的细胞，然后使用神经化学程序，表征含有这些新受体的细胞类型。使用选择性药物，其他神经递质在黄体酮作用中的作用将被确定。这些研究探讨了类固醇激素的新作用，对了解这些重要分子的生理和病理生理非常有益。