Acquisition of an Advanced Plant Molecular Genetics Facility

收购先进的植物分子遗传学设施

• 批准号: 9512366
• 负责人: Richard Michelmore
• 金额: $ 25.54万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9512366&HistoricalAwards=false
• 关键词: Acquisition; Advanced; Plant; Molecular; Genetics

We will acquire and operate instruments for a high-throughput automated plant molecular genetics facility. The facility will comprise of an automated DNA sequencer, a DNA extraction robot, a laboratory workstation, a thermocycler, a phosphoimager, and supporting computers. The facility will primarily provide three areas of research support: DNA sequencing, quantitative analysis of gene expression, and genetic analysis. The facility will impact at least 24 productive research programs in nine departments that study plant molecular genetics, ranging from fundamental molecular genetics on model species to applied crop improvement programs. We are currently severely limited by the lack of this instrumentation. All these studies would benefit from this facility. Several experiments are impossible without it. Reliable generation of accessible DNA sequence data is a fundamental component for many biological investigations, particularly gene identification and characterization. Accurate measurement of gene expression is critical to studies on gene function. Molecular markers have now been developed for most of the species under study; these should now be utilized. This facility will allow a scale of experimentation that would be otherwise impossible in an academic setting. Automated DNA extraction as well as template preparation and analysis will allow large numbers of samples to be run with accuracy. Both the UC Davis Office of Research and the College of Agriculture and Environmental Sciences are committed to developing this facility as demonstrated by their level of financial support. Their contribution, together with that from Keygene, constitutes a 50% match to the amount requested from NSF. The College will provide technical support for a total of five years, two years beyond the funding period. We will continue to develop and apply efficient molecular marker technologies. In particular, we are collaborating with Applied Biosystems to analyze Ampli fied Fragment Length Polymorphisms as codominant markers on their instruments. This will increase the utility of their machines and allow hundreds of markers to be analyzed on many individuals. It will also reduce the generation of radioactive waste.

我们将为高通量自动化植物分子遗传学设施获取和操作工具。该设施将包括一个自动DNA测序仪，DNA提取机器人，实验室工作站，热环体，磷酸可能器和支持计算机。 该设施将主要提供研究支持的三个领域：DNA测序，基因表达的定量分析和遗传分析。 该设施将在研究植物分子遗传学的九个部门中至少有24个生产研究计划，从模型物种的基本分子遗传学到应用作物改善计划的范围。目前，我们由于缺乏这种仪器而受到严重限制。所有这些研究都将从该设施中受益。没有它，几个实验是不可能的。可靠的可访问DNA序列数据是许多生物学研究，尤其是基因鉴定和表征的基本组成部分。基因表达的准确测量对于基因功能的研究至关重要。现在已经针对正在研究的大多数物种开发了分子标记。现在应该利用这些。该设施将允许在学术环境中进行一系列实验。自动DNA提取以及模板准备和分析将使大量样品准确地运行。 加州大学戴维斯分校研究办公室和农业与环境科学学院都致力于开发这种设施，如其财务支持水平所证明的那样。他们的贡献以及Keygene的贡献与NSF要求的金额构成50％的匹配。该学院将提供总计五年的技术支持，超出了资金期。 我们将继续开发和应用有效的分子标记技术。特别是，我们正在与应用生物系统合作，以分析其仪器上的附加片段长度多态性作为共同标记。这将增加机器的效用，并允许对许多人进行数百个标记。它还将减少放射性废物的产生。