Arabidopsis 2010: Functional Genomics of NBS-LRR Mediated Resistance

拟南芥 2010：NBS-LRR 介导的抗性的功能基因组学

• 批准号: 0822393
• 负责人: Richard Michelmore
• 金额: $ 249.84万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-15 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0822393&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Functional; Genomics; NBS

Proposal number IOS-0822393This project fuses genetic, evolutionary, and functional approaches to understand the co-evolution of plants and pathogens at the molecular level. Protein-protein interactions will be analyzed in yeast and in plants to build an interaction network of receptor (NBS-LRR) proteins, plant signaling components, and virulence effector proteins from bacterial and oomycete pathogens. The functional significance of these interactions to disease will be studied by a variety of cell biology and disease assays. The project will reveal whether targets of effectors represent a limited number of points of weakness in the plant that are exploited by multiple effectors and pathogens and whether protein complexes that determine the infection phenotype co-evolve. Libraries of genes encoding NBS-LRR proteins and their domains as well as signaling proteins that are candidate plant targets of effectors studied will be distributed through the Arabidopsis Biological Resource Center.The global approach will generate large amounts of data that will impact many labs focused on understanding the molecular basis of disease resistance in plants as well as contribute to the functional characterization of numerous genes in Arabidopsis. Data from the project will be broadly disseminated through the project web site (http://niblrrs.ucdavis.edu ) and through The Arabidopsis Information Resource. Deciphering the molecular determinants of resistance has practical importance as well as fundamental interest. A greater understanding of the mechanisms of perception and responses will provide new possibilities for developing disease resistant plants. Consequently, this project will expand the options available to plant breeders to achieve more durable resistance. Researchers involved in the project will benefit from inter-disciplinary training that includes the molecular genetics and cell biology of the defense response components and protein-protein interactions involved in pathogen-host interactions. Outreach efforts will include dissemination of genomics approaches to high schools through curriculum development, workshops and internships.

提案编号IOS-0822393这个项目融合了遗传，进化和功能方法，以了解分子水平上植物和病原体的共同进化。 将在酵母和植物中分析蛋白质 - 蛋白质相互作用，以建立一个受体（NBS-LRR）蛋白，植物信号成分以及来自细菌和卵形病原体的毒力效应蛋白的相互作用网络。这些相互作用与疾病的功能意义将通过多种细胞生物学和疾病分析来研究。 该项目将揭示效应子的靶标在植物中是否代表有限数量的弱点，这些弱点是由多个效应子和病原体所利用的，以及决定感染表型共同进化的蛋白质复合物是否。 编码NBS-LRR蛋白及其结构域的基因库以及所研究效应植物目标的信号传导蛋白将通过拟南芥生物资源中心分布。该全球方法将产生大量的数据，这些数据将影响许多实验室，这些数据将影响许多实验室，这些实验室专注于理解植物中疾病的分子基础，以及对动态的贡献构成了许多基因的特征。 该项目的数据将通过项目网站（http://niblrrrrrrs.ucdavis.edu）和拟南芥信息资源广泛传播。 破译抗性的分子决定因素具有实际的重要性和基本利益。 对感知和反应的机制有更深入的了解将为发展抗病植物提供新的可能性。因此，该项目将扩大植物育种者可用的选择，以实现更耐用的阻力。参与该项目的研究人员将受益于跨学科培训，其中包括防御反应成分的分子遗传学和细胞生物学以及参与病原体宿主相互作用的蛋白质 - 蛋白质相互作用。 推广工作将包括通过课程开发，研讨会和实习来传播基因组学方法。