Robotics System for Physical Mapping of Fungal Genomes

整个染色体的物理图谱在遗传学的一些根本性突破中发挥了至关重要的作用。3个模式遗传系统(黑曲霉、粗糙脉孢菌和稻瘟病菌)以及其他真菌如红球线虫的高分辨率物理图谱的完成，可能会为真菌的分子和进化遗传学研究开辟新的途径。通过构建一张29kb碱基(Kb)分辨率的多核拟青霉基因组物理图谱，我们显著地观察到多核拟青霉染色体上重复序列的分布呈现出交替的带型。这一观察结果对基因组的发育、功能组织、染色体结构和功能、重组和基因组进化具有重要意义。在这项提案中，我们计划指定并获得一个用于物理测绘的机器人系统，该系统将使几个实验室能够确定其他丝状真菌，如粗毛拟青霉和稻瘟病菌，是否在其染色体上的重复序列中共享这种带型。这个机器人系统将由佐治亚大学的真菌基因组资源(FGR)维护，并将通过提供一系列新的实验策略来解决真菌遗传学中的一大类问题，从而为整个真菌遗传学社区服务。

Physical maps of whole chromosomes have placed an essential role in a number of fundamental breakthroughs in genetics. The completion of high resolution physical maps of three model genetic systems, Aspergillus nidulans, Neurospora crassa, and Magnaporthe grisea, and of other fungi such as Nectria haematococca, is likely to open up new avenues of research on the molecular and evolutionary genetics of fungi. By building a 29 kilobase (kb) resolution physical map of the A. nidulans genome, we have made the striking observation that there is an alternating banding pattern in the distribution of repeated sequences along the chromosomes of A. nidulans. This observation has implications for development, functional organization of genomes, chromosome structure and function, recombination, and genome evolution. In this proposal we plan to specify and acquire a robotics system for physical mapping which will enable several laboratories to ascertain whether or not other filamentous fungi, such as N. crassa and M. grisea, share this banding pattern in repeated sequences along their chromosomes. This robotics system will be maintained by the Fungal Genome Resource (FGR) at the University of Georgia and will serve the fungal genetics community as a whole by providing an array of new experimental strategies to address a broad class of problems in fungal ,genetics.