Proteasomes in the Methanogenic Archea
产甲烷古菌中的蛋白酶体
基本信息
- 批准号:9513863
- 负责人:
- 金额:$ 25.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:1996
- 资助国家:美国
- 起止时间:1996-03-01 至 2000-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
9513863 Ferry Proteasomes are prevalent in the Eucarya domain where they have at least three distinct endopeptidase activities which catalyze hydrolysis of peptide bonds on the carboxyl side of hydrophobic, basic, and acidic amino acid residues. It is proposed that the eukaryotic proteasome is the "catalytic core" in a larger complex that degrades proteins labeled with ubiquitin in an ATP-dependent process. A earlier survey of species from the Bacteria and Archaea domains led to the suggestion that Thermoplasma species are the only prokaryotes containing proteasomes. Recent documentation of proteasomes in phylogenetically and physiologically diverse species of methanogenic microbes, the largest group in the Archaea, demonstrates that proteasomes are more widespread among prokaryotes than previously known. The overall focus of this work is an investigating into the physiology, biochemistry and molecular biology of protein turnover in this large group of anaerobic microbes. The specific aims of this project are: (1) biochemically characterize the Methanosarcina thermophila proteasome, and (2) address its physiological function by ascertaining the factors influencing the regulation of proteasome synthesis and activity, and determining the roles for the proteasome and ubiquitin in protein turnover. The results are expected to: (1) expand the fundamental knowledge of the evolution, mechanism and function of ubiquitin and proteasomes in all of nature, (2) provide a broader understanding of the biochemistry, genetics and physiology of M. thermophila and the methanogenic Archaea, and (3) help to further define the evolutionary relationships between the Archaea and Eucarya domains. %%% Proteasomes are complex enzyme aggregates that mediate protein breakdown in eukaryotic cells. Recently, proteasomes have been identified in archaebacteria, one of the two major groups of prokaryotes. In this project, the molecular structure of proteasomes from a methane-producing bacterium, Methanosarcina thermophila will be studied. In addition, the function of these structures will be investigated. The results will provide evidence about evolutionary relationships among major groups of organisms as well as information about how archaebacteria regulate degradation of their proteins, an unexplored area. ***
9513863 Ferry 蛋白酶体普遍存在于真核生物结构域中,它们具有至少三种不同的内肽酶活性,可催化疏水性、碱性和酸性氨基酸残基羧基侧肽键的水解。有人提出,真核蛋白酶体是较大复合物中的“催化核心”,该复合物在 ATP 依赖性过程中降解泛素标记的蛋白质。早期对细菌和古细菌领域物种的调查表明,热原体物种是唯一含有蛋白酶体的原核生物。最近对系统发育和生理学上不同的产甲烷微生物物种(古细菌中最大的类群)中蛋白酶体的记录表明,蛋白酶体在原核生物中比以前已知的更为广泛。这项工作的总体重点是研究这一大群厌氧微生物中蛋白质周转的生理学、生物化学和分子生物学。该项目的具体目标是:(1)对嗜热甲烷八叠球菌蛋白酶体进行生化表征,(2)通过确定影响蛋白酶体合成和活性调节的因素以及确定蛋白酶体和泛素在蛋白质周转中的作用来研究其生理功能。研究结果预计将:(1)扩展自然界中泛素和蛋白酶体的进化、机制和功能的基础知识,(2)提供对嗜热毁丝霉和产甲烷古菌的生物化学、遗传学和生理学更广泛的理解,以及(3)有助于进一步确定古菌和真核菌领域之间的进化关系。 %%% 蛋白酶体是介导真核细胞中蛋白质分解的复杂酶聚集体。 最近,在原核生物两大类之一的古细菌中发现了蛋白酶体。 在该项目中,将研究来自产甲烷细菌嗜热甲烷八叠球菌的蛋白酶体的分子结构。 此外,还将研究这些结构的功能。 研究结果将提供有关主要生物体之间进化关系的证据,以及有关古细菌如何调节其蛋白质降解的信息,这是一个尚未探索的领域。 ***
项目成果
期刊论文数量(0)
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会议论文数量(0)
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James Ferry其他文献
Managing Cancer Related Pain and Opioid Dependence with Sublocade: An Interdisciplinary Approach
使用Sublocade管理癌症相关疼痛和阿片类药物依赖:一种跨学科方法
- DOI:
10.1016/j.jpainsymman.2025.02.427 - 发表时间:
2025-05-01 - 期刊:
- 影响因子:3.500
- 作者:
James Ferry;Sara Happel - 通讯作者:
Sara Happel
Solvent effects on the harvesting of stratum corneum from hairless mouse skin through adhesive tape stripping in vitro
- DOI:
10.1016/0378-5173(91)90135-b - 发表时间:
1991-02-01 - 期刊:
- 影响因子:
- 作者:
Tsai Jui-Chen;Markus J. Cappel;Norman D. Weiner;Gordon L. Flynn;James Ferry - 通讯作者:
James Ferry
James Ferry的其他文献
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{{ truncateString('James Ferry', 18)}}的其他基金
MRI: Acquisition of an AB SCIEX Triple TOF5600 Spectrometer for Metabolomics Research
MRI:购买 AB SCIEX Triple TOF5600 光谱仪用于代谢组学研究
- 批准号:
1126373 - 财政年份:2011
- 资助金额:
$ 25.5万 - 项目类别:
Standard Grant
Biochemistry and Physiology of the Rnf Complex From the Archaeon Methanosarcina Acetivorans
古细菌 Methanosarcina acetivorans 的 Rnf 复合物的生物化学和生理学
- 批准号:
0820734 - 财政年份:2008
- 资助金额:
$ 25.5万 - 项目类别:
Standard Grant
Structure/Function and Physiology of Novel Enzymes from Diverse Microbes
多种微生物新型酶的结构/功能和生理学
- 批准号:
9602232 - 财政年份:1996
- 资助金额:
$ 25.5万 - 项目类别:
Continuing Grant
Biochemistry and Molecular Genetics of Formate Utilization in Methanogenic Archaebacteria
产甲烷古细菌甲酸利用的生物化学和分子遗传学
- 批准号:
9596154 - 财政年份:1995
- 资助金额:
$ 25.5万 - 项目类别:
Continuing Grant
Biochemistry and Molecular Genetics of Formate Utilization in Methanogenic Archaebacteria
产甲烷古细菌甲酸利用的生物化学和分子遗传学
- 批准号:
9106456 - 财政年份:1992
- 资助金额:
$ 25.5万 - 项目类别:
Continuing Grant
Formate Dehydrogenase- and Hydrogenase-Linked Electron Transport in Methanogenic Bacteria
产甲烷细菌中甲酸脱氢酶和氢化酶相关的电子传递
- 批准号:
8409558 - 财政年份:1984
- 资助金额:
$ 25.5万 - 项目类别:
Continuing Grant
Hydrogenase - and Formate Dehydrogenase - Linked Electron Transport in Methanogenic Bacteria
产甲烷细菌中氢化酶和甲酸脱氢酶相关的电子传递
- 批准号:
7812148 - 财政年份:1979
- 资助金额:
$ 25.5万 - 项目类别:
Standard Grant
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