Control of Cellular Differentiation in Symbiotically Competent Cyanobacteria
共生蓝藻细胞分化的控制
基本信息
- 批准号:9514787
- 负责人:
- 金额:$ 33万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:1996
- 资助国家:美国
- 起止时间:1996-02-01 至 2000-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
*** 9514787 Meeks The long-term goal of this program is to identify, characterize and manipulate genes involved in cellular differentiation in the cyanobacterium Nostoc sp. strain ATCC 29133, a strain that can differentiate motile hormogonial filaments, akinetes (spores) and heterocysts, which are the sites of N2 fixation. Nostoc 29133 is also a facultative heterotroph that can establish a symbiotic association, via hormogonia, with the bryophyte Anthoceros punctatus. Anthoceros produces extracellular metabolites that sequentially stimulate hormogonia and heterocyst differentiation of Nostoc spp. to 10-fold or higher levels than those seen in its absence. The physiological and molecular genetic techniques developed in the prior grant and the transposon mutants currently in hand form the methodological and biological foundation of this proposal. Specific objectives are proposed in the following two areas. I . Heterocyst maturation is dependent on a signal cascade regulatory system. Analysis of Nostoc 29133: :Tn5-1063 strain UCD 311 (Fox~Sym+) with an insertion devR, encoding a receiver-domain-only response regulator protein. Biochemical and genetic assays to identify potential phosphodonors and phosphoreceivers of DevR. This project will identify members of a two component regulatory system involved in heterocyst development. 2. Hormogonia differentiation is a consequence of differential gene expression. Establishment and analysis of a hormogonia-specific subtractive cDNA library; generation by transposition of mutants of Nostoc 29133 altered in hormogonia formation; genetic characterization of such mutants and of cosmic clones from cDNA analysis. This project is the initial effort in understanding how cells divide uncoupled from DNA replication and biomass increase to form hormogonia and then return to the vegetative growth state in completing a cellular life cycle. ***
*** 9514787米克斯 该计划的长期目标是鉴定、表征和操纵蓝细菌Nostoc sp.菌株ATCC 29133中参与细胞分化的基因,该菌株可以分化能动的藻殖体丝、动丝(孢子)和异形胞,这些是N2固定的位点。念珠藻29133也是一种兼性异养生物,可以通过藻殖体与斑角蝇(Anthoceros punctatus)建立共生关系。Anthoceros产生胞外代谢产物,依次刺激念珠藻的藻殖子和异形胞分化。到10倍或更高的水平比那些在它的缺席。在先前的赠款和转座子突变体目前在手的生理和分子遗传技术开发形成的方法和生物学基础,这一建议。在以下两个领域提出了具体目标。I .异形胞成熟依赖于信号级联调节系统。具有插入devR的念珠藻29133::Tn 5 -1063菌株UCD 311(Fox~Sym+)的分析,所述插入devR编码仅受体结构域的应答调节蛋白。生物化学和遗传测定,以确定潜在的磷酸供体和磷酸受体的DevR。这个项目将确定一个两个组件的调控系统的成员参与异形胞的发展。2.激素生殖器分化是基因表达差异的结果。藻殖体特异性消减cDNA文库的建立和分析;通过转座产生藻殖体形成改变的念珠藻29133突变体;从cDNA分析中对这些突变体和宇宙克隆进行遗传表征。该项目是了解细胞如何从DNA复制和生物量增加中分离出来,形成藻殖体,然后恢复到营养生长状态,完成细胞生命周期的初步努力。 ***
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
John Meeks其他文献
John Meeks的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('John Meeks', 18)}}的其他基金
Collaborative Research: Dimensions: Integrating phylogenetics, ecophysiology and transcriptomics to understand the diversity of hornwort-cyanobacterium symbiosis.
合作研究:维度:整合系统发育学、生态生理学和转录组学,了解金鱼藻-蓝藻共生的多样性。
- 批准号:
1831552 - 财政年份:2019
- 资助金额:
$ 33万 - 项目类别:
Standard Grant
The roles of chemotaxis-like signal transduction systems in the differentiation and behavior of motile filaments of the cyanobacterium Nostoc punctiforme
趋化样信号转导系统在点状发菜蓝藻运动丝分化和行为中的作用
- 批准号:
0822008 - 财政年份:2008
- 资助金额:
$ 33万 - 项目类别:
Continuing Grant
Conference: Annual Meetings of the West Coast Bacterial Physiologists to be held on the West Coast of the USA in mid December
会议:西海岸细菌生理学家年会将于 12 月中旬在美国西海岸举行
- 批准号:
0750212 - 财政年份:2007
- 资助金额:
$ 33万 - 项目类别:
Standard Grant
Emerging Frontier 03: Functional Analyses of the Genome of the Phenotypically Complex, Multicellular Cyanobacterium Nostoc Punctiforme
新兴前沿 03:表型复杂的多细胞蓝藻发菜基因组的功能分析
- 批准号:
0317104 - 财政年份:2003
- 资助金额:
$ 33万 - 项目类别:
Standard Grant
Conference: Annual Meetings of the West Coast Bacterial Physiologists, to be held in Pacific Grove, California, December, 2002
会议:西海岸细菌生理学家年会,将于 2002 年 12 月在加利福尼亚州太平洋丛林举行
- 批准号:
0233365 - 财政年份:2002
- 资助金额:
$ 33万 - 项目类别:
Continuing Grant
Control of Hormogonium Differentiation in the Symbiotic Cyanobacterium Nostoc Punctiforme
共生蓝藻点状发菜中激素分化的控制
- 批准号:
0080805 - 财政年份:2000
- 资助金额:
$ 33万 - 项目类别:
Continuing Grant
Regulation of Glucose-6-Phosphate Dehydrogenase Expression in Nostoc Punctiforme
发菜中葡萄糖-6-磷酸脱氢酶表达的调控
- 批准号:
9604270 - 财政年份:1997
- 资助金额:
$ 33万 - 项目类别:
Continuing Grant
Control of Heterocyst Differentiation in Free-Living and Symbiotic Cyanobacteria
自由生活和共生蓝藻异囊分化的控制
- 批准号:
9206130 - 财政年份:1992
- 资助金额:
$ 33万 - 项目类别:
Continuing Grant
Control of Heterocyst Differentiation in Free-Living and Symbiotic Cyanobacteria
自由生活和共生蓝藻异囊分化的控制
- 批准号:
8902204 - 财政年份:1989
- 资助金额:
$ 33万 - 项目类别:
Continuing Grant
Control of Heterocyst Differentiation in Free-Living and Symbiotic Cyanobacteria
自由生活和共生蓝藻异囊分化的控制
- 批准号:
8608494 - 财政年份:1986
- 资助金额:
$ 33万 - 项目类别:
Standard Grant
相似国自然基金
Cellular & Molecular Immunology
- 批准号:30824806
- 批准年份:2008
- 资助金额:20.0 万元
- 项目类别:专项基金项目
相似海外基金
Elucidation and improved control of human induced pluripotent stem cell cardiac differentiation by using single-guide RNA-based cellular barcoding to track and manipulate lineages
通过使用基于单向导 RNA 的细胞条形码来跟踪和操纵谱系,阐明并改进对人类诱导多能干细胞心脏分化的控制
- 批准号:
10752369 - 财政年份:2023
- 资助金额:
$ 33万 - 项目类别:
Elucidation of high gravity-sensing mechanisms in cells and its application to control cellular phenotype and differentiation
阐明细胞中的高重力感应机制及其在控制细胞表型和分化中的应用
- 批准号:
22K19898 - 财政年份:2022
- 资助金额:
$ 33万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Elucidating Cellular Aging and Quality Control Pathways through Meiotic Differentiation
通过减数分裂分化阐明细胞衰老和质量控制途径
- 批准号:
10469001 - 财政年份:2021
- 资助金额:
$ 33万 - 项目类别:
Elucidating Cellular Aging and Quality Control Pathways through Meiotic Differentiation
通过减数分裂分化阐明细胞衰老和质量控制途径
- 批准号:
10299523 - 财政年份:2021
- 资助金额:
$ 33万 - 项目类别:
Elucidating Cellular Aging and Quality Control Pathways through Meiotic Differentiation
通过减数分裂分化阐明细胞衰老和质量控制途径
- 批准号:
10657538 - 财政年份:2021
- 资助金额:
$ 33万 - 项目类别:
Control of cellular differentiation in the developing brain
大脑发育中细胞分化的控制
- 批准号:
DP200102363 - 财政年份:2020
- 资助金额:
$ 33万 - 项目类别:
Discovery Projects
Metabolic control of cellular differentiation and the fibrotic response
细胞分化和纤维化反应的代谢控制
- 批准号:
10094236 - 财政年份:2019
- 资助金额:
$ 33万 - 项目类别:
Molecular and cellular control of human Th9 cell differentiation in health and disease
健康和疾病中人类 Th9 细胞分化的分子和细胞控制
- 批准号:
nhmrc : GNT1138359 - 财政年份:2018
- 资助金额:
$ 33万 - 项目类别:
Project Grants
Structure elucidation of developmental-stage selective cell function regulators that control cellular differentiation
控制细胞分化的发育阶段选择性细胞功能调节剂的结构阐明
- 批准号:
18K05335 - 财政年份:2018
- 资助金额:
$ 33万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular and cellular control of human Th9 cell differentiation in health and disease
健康和疾病中人类 Th9 细胞分化的分子和细胞控制
- 批准号:
nhmrc : 1138359 - 财政年份:2018
- 资助金额:
$ 33万 - 项目类别:
Project Grants