Control of Cellular Differentiation in Symbiotically Competent Cyanobacteria

共生蓝藻细胞分化的控制

基本信息

  • 批准号:
    9514787
  • 负责人:
  • 金额:
    $ 33万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    1996
  • 资助国家:
    美国
  • 起止时间:
    1996-02-01 至 2000-07-31
  • 项目状态:
    已结题

项目摘要

*** 9514787 Meeks The long-term goal of this program is to identify, characterize and manipulate genes involved in cellular differentiation in the cyanobacterium Nostoc sp. strain ATCC 29133, a strain that can differentiate motile hormogonial filaments, akinetes (spores) and heterocysts, which are the sites of N2 fixation. Nostoc 29133 is also a facultative heterotroph that can establish a symbiotic association, via hormogonia, with the bryophyte Anthoceros punctatus. Anthoceros produces extracellular metabolites that sequentially stimulate hormogonia and heterocyst differentiation of Nostoc spp. to 10-fold or higher levels than those seen in its absence. The physiological and molecular genetic techniques developed in the prior grant and the transposon mutants currently in hand form the methodological and biological foundation of this proposal. Specific objectives are proposed in the following two areas. I . Heterocyst maturation is dependent on a signal cascade regulatory system. Analysis of Nostoc 29133: :Tn5-1063 strain UCD 311 (Fox~Sym+) with an insertion devR, encoding a receiver-domain-only response regulator protein. Biochemical and genetic assays to identify potential phosphodonors and phosphoreceivers of DevR. This project will identify members of a two component regulatory system involved in heterocyst development. 2. Hormogonia differentiation is a consequence of differential gene expression. Establishment and analysis of a hormogonia-specific subtractive cDNA library; generation by transposition of mutants of Nostoc 29133 altered in hormogonia formation; genetic characterization of such mutants and of cosmic clones from cDNA analysis. This project is the initial effort in understanding how cells divide uncoupled from DNA replication and biomass increase to form hormogonia and then return to the vegetative growth state in completing a cellular life cycle. ***
*9514787 Meek该计划的长期目标是识别、表征和操纵蓝藻发菜中与细胞分化有关的基因。ATCC 29133菌株是一株能区分动态丝、无动体(孢子)和异形胞的菌株,它们是固定氮气的部位。发菜29133也是一种兼性异养菌,可以通过配子体与苔藓植物花斑苔藓建立共生关系。花生菌产生胞外代谢产物,进而刺激念珠藻的异形胞和异形胞的分化。到10倍或更高的水平比在没有它的情况下看到的。在先前的资助中开发的生理和分子遗传技术以及目前手中的转座子突变体构成了这一提议的方法学和生物学基础。在以下两个方面提出了具体目标。我..。异型囊泡的成熟依赖于信号级联调控系统。Nostoc 29133::Tn5-1063株UCD311(Fox~Sym+)插入DevR的分析,编码仅受体域的反应调节蛋白。生化和遗传分析,以确定潜在的磷和磷受体的DevR。该项目将确定参与杂胞体发育的两组分调控系统的成员。2.精原细胞分化是基因表达差异的结果。原神经节特异消减文库的建立和分析;原生孢子形成过程中发生突变的发菜29133突变体的转座产生;这些突变体和宇宙克隆的遗传特征。该项目是了解细胞如何从DNA复制和生物量增加解偶联分裂形成原生细胞,然后在完成细胞生命周期后返回营养生长状态的初步努力。***

项目成果

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John Meeks其他文献

John Meeks的其他文献

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{{ truncateString('John Meeks', 18)}}的其他基金

Collaborative Research: Dimensions: Integrating phylogenetics, ecophysiology and transcriptomics to understand the diversity of hornwort-cyanobacterium symbiosis.
合作研究:维度:整合系统发育学、生态生理学和转录组学,了解金鱼藻-蓝藻共生的多样性。
  • 批准号:
    1831552
  • 财政年份:
    2019
  • 资助金额:
    $ 33万
  • 项目类别:
    Standard Grant
The roles of chemotaxis-like signal transduction systems in the differentiation and behavior of motile filaments of the cyanobacterium Nostoc punctiforme
趋化样信号转导系统在点状发菜蓝藻运动丝分化和行为中的作用
  • 批准号:
    0822008
  • 财政年份:
    2008
  • 资助金额:
    $ 33万
  • 项目类别:
    Continuing Grant
Conference: Annual Meetings of the West Coast Bacterial Physiologists to be held on the West Coast of the USA in mid December
会议:西海岸细菌生理学家年会将于 12 月中旬在美国西海岸举行
  • 批准号:
    0750212
  • 财政年份:
    2007
  • 资助金额:
    $ 33万
  • 项目类别:
    Standard Grant
Emerging Frontier 03: Functional Analyses of the Genome of the Phenotypically Complex, Multicellular Cyanobacterium Nostoc Punctiforme
新兴前沿 03:表型复杂的多细胞蓝藻发菜基因组的功能分析
  • 批准号:
    0317104
  • 财政年份:
    2003
  • 资助金额:
    $ 33万
  • 项目类别:
    Standard Grant
Conference: Annual Meetings of the West Coast Bacterial Physiologists, to be held in Pacific Grove, California, December, 2002
会议:西海岸细菌生理学家年会,将于 2002 年 12 月在加利福尼亚州太平洋丛林举行
  • 批准号:
    0233365
  • 财政年份:
    2002
  • 资助金额:
    $ 33万
  • 项目类别:
    Continuing Grant
Control of Hormogonium Differentiation in the Symbiotic Cyanobacterium Nostoc Punctiforme
共生蓝藻点状发菜中激素分化的控制
  • 批准号:
    0080805
  • 财政年份:
    2000
  • 资助金额:
    $ 33万
  • 项目类别:
    Continuing Grant
Regulation of Glucose-6-Phosphate Dehydrogenase Expression in Nostoc Punctiforme
发菜中葡萄糖-6-磷酸脱氢酶表达的调控
  • 批准号:
    9604270
  • 财政年份:
    1997
  • 资助金额:
    $ 33万
  • 项目类别:
    Continuing Grant
Control of Heterocyst Differentiation in Free-Living and Symbiotic Cyanobacteria
自由生活和共生蓝藻异囊分化的控制
  • 批准号:
    9206130
  • 财政年份:
    1992
  • 资助金额:
    $ 33万
  • 项目类别:
    Continuing Grant
Control of Heterocyst Differentiation in Free-Living and Symbiotic Cyanobacteria
自由生活和共生蓝藻异囊分化的控制
  • 批准号:
    8902204
  • 财政年份:
    1989
  • 资助金额:
    $ 33万
  • 项目类别:
    Continuing Grant
Control of Heterocyst Differentiation in Free-Living and Symbiotic Cyanobacteria
自由生活和共生蓝藻异囊分化的控制
  • 批准号:
    8608494
  • 财政年份:
    1986
  • 资助金额:
    $ 33万
  • 项目类别:
    Standard Grant

相似国自然基金

Cellular & Molecular Immunology
  • 批准号:
    30824806
  • 批准年份:
    2008
  • 资助金额:
    20.0 万元
  • 项目类别:
    专项基金项目

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Elucidation and improved control of human induced pluripotent stem cell cardiac differentiation by using single-guide RNA-based cellular barcoding to track and manipulate lineages
通过使用基于单向导 RNA 的细胞条形码来跟踪和操纵谱系,阐明并改进对人类诱导多能干细胞心脏分化的控制
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