Emerging Frontier 03: Functional Analyses of the Genome of the Phenotypically Complex, Multicellular Cyanobacterium Nostoc Punctiforme

新兴前沿 03：表型复杂的多细胞蓝藻发菜基因组的功能分析

• 批准号: 0317104
• 负责人: John Meeks
• 金额: --
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0317104&HistoricalAwards=false
• 关键词: Emerging; Frontier; 03; Functional; Analyses

The experimental core of this project is functional genomic analyses of the cyanobacterium Nostoc punctiforme. The shotgun sequencing of the 9.25 Mbp genome of N. punctiforme has been completed and an annotated database is available at http://www.jgi.doe.gov . N. punctiforme has an extraordinarily wide range of ecological niches, physiological properties and cellular developmental alternatives, including the unusual ability of fixing nitrogen in both free-living and plant-associated symbiotic growth states. Nitrogen fixation occurs in terminally differentiated cells called heterocysts. In addition to heterocysts, N. punctiforme vegetative cells can transiently differentiate into resting cells termed akinetes and motile filaments called hormogonia. The working hypothesis is that the regulatory elements and pathways governing cellular differentiation alternatives and symbiotic interactions of N. punctiforme uniquely evolved in cyanobacteria. Therefore, functional, in addition to computational, analyses are required to identify the developmental genetic elements and regulatory circuits present in the genome. Understanding how these circuits are integrated in N. punctiforme has implications on the evolution of developmental pathways in complex organisms. This project will utilize high throughput genomic methodologies of global gene expression assays, using DNA microarrays, and mutagenesis. The resources generated, including all of the individually amplified genes present in the genome, will be made available to the national and international cyanobacterial community. Researchers at the level of principle investigators, technicians, postdoctoral fellows, graduate and undergraduate students, will interact in developing and applying these new methodologies to this complex cyanobacterium; thus, the project affords an integrative training opportunity.

本计画的实验核心是蓝细菌点状念珠藻的功能基因组分析。对N. punctiforme已经完成，并在http://www.jgi.doe.gov上提供了一个附有注释的数据库。N. punctiforme具有非常广泛的生态位、生理特性和细胞发育选择，包括在自由生活和与植物相关的共生生长状态下固定氮的不寻常能力。固氮作用发生在被称为异形胞的终末分化细胞中。除异形胞外，N.点状营养细胞可短暂分化为称为动丝的静止细胞和称为藻殖体的运动丝。工作假设是，控制细胞分化的调控元件和途径的选择和共生相互作用的N。在蓝细菌中进化出独特的点状结构。因此，功能，除了计算，需要分析，以确定基因组中存在的发育遗传元件和调控电路。了解这些电路是如何在N. punctiforme对复杂生物体发育途径的进化具有重要意义。该项目将利用高通量基因组方法学的全球基因表达测定，使用DNA微阵列和诱变。所产生的资源，包括基因组中存在的所有单独扩增的基因，将提供给国家和国际蓝藻社区。研究人员在原则调查员，技术人员，博士后研究员，研究生和本科生的水平，将在开发和应用这些新的方法，这种复杂的蓝藻互动;因此，该项目提供了一个综合培训的机会。