Emerging Frontier 03: Functional Analyses of the Genome of the Phenotypically Complex, Multicellular Cyanobacterium Nostoc Punctiforme

新兴前沿 03：表型复杂的多细胞蓝藻发菜基因组的功能分析

• 批准号: 0317104
• 负责人: John Meeks
• 金额: --
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0317104&HistoricalAwards=false
• 关键词: Emerging; Frontier; 03; Functional; Analyses

The experimental core of this project is functional genomic analyses of the cyanobacterium Nostoc punctiforme. The shotgun sequencing of the 9.25 Mbp genome of N. punctiforme has been completed and an annotated database is available at http://www.jgi.doe.gov . N. punctiforme has an extraordinarily wide range of ecological niches, physiological properties and cellular developmental alternatives, including the unusual ability of fixing nitrogen in both free-living and plant-associated symbiotic growth states. Nitrogen fixation occurs in terminally differentiated cells called heterocysts. In addition to heterocysts, N. punctiforme vegetative cells can transiently differentiate into resting cells termed akinetes and motile filaments called hormogonia. The working hypothesis is that the regulatory elements and pathways governing cellular differentiation alternatives and symbiotic interactions of N. punctiforme uniquely evolved in cyanobacteria. Therefore, functional, in addition to computational, analyses are required to identify the developmental genetic elements and regulatory circuits present in the genome. Understanding how these circuits are integrated in N. punctiforme has implications on the evolution of developmental pathways in complex organisms. This project will utilize high throughput genomic methodologies of global gene expression assays, using DNA microarrays, and mutagenesis. The resources generated, including all of the individually amplified genes present in the genome, will be made available to the national and international cyanobacterial community. Researchers at the level of principle investigators, technicians, postdoctoral fellows, graduate and undergraduate students, will interact in developing and applying these new methodologies to this complex cyanobacterium; thus, the project affords an integrative training opportunity.

该项目的实验核心是对Nostoc点状细菌的功能基因组分析。 N. Punctiforme的9.25 MBP基因组的shot弹枪测序已完成，并在http://www.jgi.doe.gov上获得了注释数据库。 N. Punctiforme具有非常广泛的生态壁ches，生理特性和细胞发育替代方案，包括在自由生活和植物相关的共生生长状态下固定氮的异常能力。氮固定发生在称为杂环的终末分化细胞中。除杂囊外，N。unctiforme植物细胞还可以瞬时分化为称为Akinetes的静息细胞和称为荷尔蒙病的通风丝。有效的假设是，在蓝细菌中唯一进化的细胞分化替代方案和共生相互作用的调节元件和途径。因此，除了计算外，还需要进行功能，以确定基因组中存在的发育遗传元件和调节回路。了解这些电路是如何整合到点状的，对复杂生物体中发育途径的演变有影响。该项目将使用DNA微阵列和诱变，利用全球基因表达测定法的高通量基因组方法。所产生的资源，包括基因组中存在的所有单独放大基因，将提供给国家和国际蓝细菌群落。主要研究人员，技术人员，博士后研究员，毕业生和本科生的研究人员将在开发和应用这些新方法中进行互动；因此，该项目提供了一个综合培训机会。