Emerging Frontier 03: Functional Analyses of the Genome of the Phenotypically Complex, Multicellular Cyanobacterium Nostoc Punctiforme

新兴前沿 03：表型复杂的多细胞蓝藻发菜基因组的功能分析

• 批准号: 0317104
• 负责人: John Meeks
• 金额: --
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0317104&HistoricalAwards=false
• 关键词: Emerging; Frontier; 03; Functional; Analyses

The experimental core of this project is functional genomic analyses of the cyanobacterium Nostoc punctiforme. The shotgun sequencing of the 9.25 Mbp genome of N. punctiforme has been completed and an annotated database is available at http://www.jgi.doe.gov . N. punctiforme has an extraordinarily wide range of ecological niches, physiological properties and cellular developmental alternatives, including the unusual ability of fixing nitrogen in both free-living and plant-associated symbiotic growth states. Nitrogen fixation occurs in terminally differentiated cells called heterocysts. In addition to heterocysts, N. punctiforme vegetative cells can transiently differentiate into resting cells termed akinetes and motile filaments called hormogonia. The working hypothesis is that the regulatory elements and pathways governing cellular differentiation alternatives and symbiotic interactions of N. punctiforme uniquely evolved in cyanobacteria. Therefore, functional, in addition to computational, analyses are required to identify the developmental genetic elements and regulatory circuits present in the genome. Understanding how these circuits are integrated in N. punctiforme has implications on the evolution of developmental pathways in complex organisms. This project will utilize high throughput genomic methodologies of global gene expression assays, using DNA microarrays, and mutagenesis. The resources generated, including all of the individually amplified genes present in the genome, will be made available to the national and international cyanobacterial community. Researchers at the level of principle investigators, technicians, postdoctoral fellows, graduate and undergraduate students, will interact in developing and applying these new methodologies to this complex cyanobacterium; thus, the project affords an integrative training opportunity.

该项目的实验核心是蓝藻点状发菜的功能基因组分析。 N. punctiforme 9.25 Mbp 基因组的鸟枪法测序已经完成，带注释的数据库可在 http://www.jgi.doe.gov 上获取。点状猪笼草具有极其广泛的生态位、生理特性和细胞发育选择，包括在自由生活和与植物相关的共生生长状态下固氮的不寻常能力。固氮发生在称为异形细胞的终末分化细胞中。除了异形细胞外，点状 N. punctiforme 营养细胞可以短暂分化为称为 akinetes 的静息细胞和称为 hormogonia 的运动细丝。工作假设是，控制细胞分化替代方案和点状奈瑟菌共生相互作用的调控元件和途径在蓝藻中独特地进化。因此，除了计算之外，还需要进行功能分析来识别基因组中存在的发育遗传元件和调控电路。了解这些回路如何在点状猪笼草中整合，对于复杂生物体发育途径的进化具有重要意义。该项目将利用高通量基因组方法进行全局基因表达测定，使用 DNA 微阵列和诱变。产生的资源，包括基因组中存在的所有单独扩增的基因，将提供给国家和国际蓝藻界。主要研究人员、技术人员、博士后研究员、研究生和本科生等级别的研究人员将互动，开发这些新方法并将其应用于这种复杂的蓝细菌；因此，该项目提供了综合培训机会。